Objective To understand the contamination status of Campylobacter in meat products of Baotou,the pathogenic characteristics such as drug resistance genes,antibiotic sensitivities,molecular typing were analyzed.Methods Three hundred meat products were collected from 2019 to 2022,which were cultured for Campylobacter isolation.Sensitivities to antibiotics,drug resistance genes and mutation sites of isolated Campylobacter were detected and genetic characteristics were analyzed by pulsed field gel electrophoresis(PFGE)method as well.Results A total of 142 Campylobacter were isolated from 300 meat products,of which the isolation rate of raw poultry was 65.71％(138/210).The detection rate of Campylobacter coli was significantly higher than that of Campylobacter jejuni(x2=12.48,P＜0.01).Only 3 strains were sensitive to all 11 antibiotics,and drug resistance were mainly manifested in quinolones(naphthalenic acid,ciprofloxacin)and tetracycline.26 strains were resistant to all 11 antibiotics and 130 strains were resistant to multiple antibiotics.Mutations in C-257-T site of gyrA gene had occyred in quinolone-resistant campylobacter.And tetO gene was detected in 91.09％of tetracycline-resistant strains.All Campylobacter could be divided into 105 PFGE types.The similarity coefficients of different PFGE types ranged from 35.1％to 100％and there was no a dominant PFGE type.Conclusion Campylobacter pollution in meat products in Baotou have been detected,and the pollution is particularly serious in raw poultry products.Campylobacter has a high degree of resistance to quinolones and tetracycline antibiotics,and the PFGE types are polymorphic.
Meat and meat productsCampylobacterdrug resistance genesdrug resistancepulsed field gel electrophoresisfoodborne bacterial pathogens
NETL
NSTL
万方数据
