2019-2022年包头市肉及肉制品中弯曲菌监测结果及病原学特征分析
Surveillance and pathogenic characteristics of Campylobacter isolated from meat products in Baotou City from 2019 to 2022
庞梦婷 1彭景贤 1薄力 1高蒙 1刘欣 1陈凯旋1
作者信息
- 1. 包头市疾病预防控制中心,内蒙古包头 014030
- 折叠
摘要
目的 了解2019-2022年包头市市售肉及肉制品中弯曲菌(Campylobacter)带菌率,并对其分离菌株的抗生素敏感性、耐药基因及分子分型等病原学特征进行研究.方法 2019-2022年共采集市售肉及肉制品300份,分离培养获得弯曲菌,测定其抗生素敏感性、耐药基因及基因突变位点,并通过脉冲场凝胶电泳(PFGE)对菌株进行分子分型.结果 300份肉及肉制品中共检出弯曲菌142株,其中生禽肉中检出率为65.71％(138/210),结肠弯曲菌检出率显著高于空肠弯曲菌(x2=12.48,P＜0.01);仅有3株弯曲菌对全部11种抗生素敏感,有26株细菌耐受全部11种抗生素并有130株发生多重抗生素耐药,主要耐药性表现在喹诺酮(萘啶酸、环丙沙星)及四环素类药物;在喹诺酮耐药弯曲菌中均检测到gyrA基因发生C-243-T的位点突变,并在91.09％的四环素耐药弯曲菌中检测到tetO耐药基因;142株弯曲菌分为105个PFGE型别,相似度在35.1％～100％,无明显优势型别条带.结论 包头市肉及肉制品中存在弯曲菌污染,且在生禽肉中污染尤为严重;弯曲菌对喹诺酮及四环素类抗生素耐药程度高且多重耐药情况较为严重,PFGE型别呈多态性.
Abstract
Objective To understand the contamination status of Campylobacter in meat products of Baotou,the pathogenic characteristics such as drug resistance genes,antibiotic sensitivities,molecular typing were analyzed.Methods Three hundred meat products were collected from 2019 to 2022,which were cultured for Campylobacter isolation.Sensitivities to antibiotics,drug resistance genes and mutation sites of isolated Campylobacter were detected and genetic characteristics were analyzed by pulsed field gel electrophoresis(PFGE)method as well.Results A total of 142 Campylobacter were isolated from 300 meat products,of which the isolation rate of raw poultry was 65.71％(138/210).The detection rate of Campylobacter coli was significantly higher than that of Campylobacter jejuni(x2=12.48,P＜0.01).Only 3 strains were sensitive to all 11 antibiotics,and drug resistance were mainly manifested in quinolones(naphthalenic acid,ciprofloxacin)and tetracycline.26 strains were resistant to all 11 antibiotics and 130 strains were resistant to multiple antibiotics.Mutations in C-257-T site of gyrA gene had occyred in quinolone-resistant campylobacter.And tetO gene was detected in 91.09％of tetracycline-resistant strains.All Campylobacter could be divided into 105 PFGE types.The similarity coefficients of different PFGE types ranged from 35.1％to 100％and there was no a dominant PFGE type.Conclusion Campylobacter pollution in meat products in Baotou have been detected,and the pollution is particularly serious in raw poultry products.Campylobacter has a high degree of resistance to quinolones and tetracycline antibiotics,and the PFGE types are polymorphic.
关键词
肉及肉制品/弯曲菌/耐药基因/耐药性/脉冲场凝胶电泳/食源性致病菌Key words
Meat and meat products/Campylobacter/drug resistance genes/drug resistance/pulsed field gel electrophoresis/foodborne bacterial pathogens引用本文复制引用
基金项目
包头市青年创新人才项目(2021102530)
包头市卫生健康科技计划(wsjkkj063)
出版年
2024
NETL
NSTL
万方数据