首页|一株毒杀松材线虫的解淀粉芽孢杆菌活性物质的基本性质

一株毒杀松材线虫的解淀粉芽孢杆菌活性物质的基本性质

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从马尾松林中筛选出的解淀粉芽孢杆菌Bacillus amyloliquefaciens JK-JS3菌株对松材线虫Bursaphelenchus xylophilus具有较高杀线活性.本试验对JK-JS3菌株分泌的杀线活性物质的基本性质进行了研究.结果表明:该菌株产生的杀线组分是可随水分一同蒸发的非蛋白质类物质;其吸收峰在190nm到200nm之间;常温25℃和冷藏4℃下保存60d仍能保持杀线活性;不同温度和pH条件对其杀线组分的活性有影响,加热会降低其活性,碱性条件下具有较高的杀线活性;石油醚、氯仿、乙酸乙酯等有机溶剂不能将其活性物质从粗提液中提取.
Elementary Properties of the Nematicidal Crude Toxin Produced by Bacillus amyloliquefaciens JK-JS3
Bacillus amyloliquefaciens JK-JS3, isolated from the leaf of the Pinus massoniana in Mount Zijin, has high nematicidal activity to Bursaphelenchus xylophilus. In order to isolate and purify the ne-maticidal components, the elementary characteristics of the nematicidal crude toxin have been studied in our experiment. The results indicated that the nematicidal toxin is not a protein which can be evaporated with the water and its maximum optical absorption is from 190 to 220 nm. The nematicidal activity of the toxin can sustain for a long period at 25 ℃ and 4 ℃. High temperature and pH condition can affect its nematicidal activity. It has strong nematicidal activity at the alkalinity condition. The nematicidal components can not be dissolved in the organic solvents such as petroleum ether, chloroform and ethyl acetate.

Bursaphelenchus xylophilusBacillus amyloliquefaciensnematicidal activityelementary property

朱丽梅、吴小芹、陈江天、徐旭凌

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南京林业大学森林资源与环境学院/江苏省有害生物预防与控制重点实验室,南京,210037

金陵科技学院园艺学院

松材线虫 解淀粉芽孢杆菌 杀线活性 基本性质

国家林业局948引进项目

2003-4-38

2009

中国生物防治学报
中国农业科学院植物保护研究所 中国植物保护学会

中国生物防治学报

CSTPCDCSCD北大核心
影响因子:1.054
ISSN:2095-039X
年,卷(期):2009.25(4)
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