首页|木霉菌协同杀菌剂防治北苍术白绢病的增效作用

木霉菌协同杀菌剂防治北苍术白绢病的增效作用

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为了提高木霉菌和杀菌剂协同对北苍术白绢病的防治效果,减少杀菌剂的使用量,本文采用生长速率法测定了 12 种杀菌剂对齐整小核菌及其拮抗木霉菌株的EC50,并采用平板对峙法和协同系数法筛选出与 3种木霉菌具有生物相容性和增效作用的杀菌剂后,测定杀菌剂与木霉菌协同对北苍术白绢病的田间防治效果.结果表明:240 g/L噻呋酰胺SC、30%噁霉灵AS和 80%代森锰锌WP与棘孢木霉T16、深绿木霉T15 和哈茨木霉 T11 间的 VEC50 均大于 1,具有良好的生物相容性.噻呋酰胺与棘孢木霉和深绿木霉协同对北苍术白绢病的田间防治效果分别达到了 80.76%和 78.82%,比噻呋酰胺的防治效果分别增长了 13.36%和10.64%.由此可见,在减少化学杀菌剂使用量时,棘孢木霉 T16 和深绿木霉 T15 均可与噻呋酰胺协同防治北苍术白绢病且具有增效作用.
Synergistic Effect of Trichoderma and Fungicides on Atractylodes chinensis Southern Blight Caused by Sclerotium rolfsii
In order to control effectively southern blight caused by Sclerotium rolfsii of Atractylodes chinensis as well as reducing the dosage of chemical fungicides,Trichoderma strains that can be used in combination with fungicides were screened out.Toxicity of 12 chemical fungicides agents and 7 Trichoderma stains against S.rolfsii was firstly tested in vitro with toxic medium method.And Trichoderma asperellum T16,Trichoderma atroviride T15 and Trichoderma harzianum T11 were subsequently shown to exhibit bio-compatibility with fungicides proved by dural culture and synergistic effect method.Control efficacy of the combination on suppressing of southern blight of A.chinensis was further tested in field experiment.The results showed that the VEC50 between 240 g/L thifluzamide SC,30%hymexazol AS and 80%mancozeb WP with T.asperellum T16,T.atroviride T15 and T.harzianum T11 were higher than 1,respectively,which indicated good bio-compatibility.In the reducing half dosage of thifluzamide condition,the synergistic control effect of thifluzamide combined with T.asperellum T16 and T.atroviride T15 on southern blight reached 80.76%and 78.82%,respectively,which increased by 13.36%and 10.64%compared with thifluzamide control.In conclusion,T.asperellum T16 and T.atroviride T15 could synergize with thifluzamide to control A.chinensis southern blight,which was much more effective than applying 100%fungicides.

Atractylodes chinensis southern blight diseaseTrichoderma asperellumTrichoderma atroviridethifluzamidesynergistic effect

贺字典、韩亚梅、金歌、高玉峰、闵康

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河北科技师范学院农学与生物科技学院/河北省作物逆境生物学重点实验室,秦皇岛 066600

河北科技师范学院学报编辑部,秦皇岛 066600

北苍术白绢病 棘孢木霉 深绿木霉 噻呋酰胺 协同作用

河北省重点研发项目河北省重点研发项目河北省自然科学基金秦皇岛市重点研发项目河北省研究生创新资助项目

21326516D22326501DC2019407103201901B022CXZZ202206

2023

中国生物防治学报
中国农业科学院植物保护研究所 中国植物保护学会

中国生物防治学报

CSTPCDCSCD北大核心
影响因子:1.054
ISSN:2095-039X
年,卷(期):2023.39(5)
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