冻土毛霉菌株BO-1对韭菜迟眼蕈蚊的致病力及防治应用评价
Pathogenicity and field control efficiency of Mucor hiemalis BO-1 against Bradysia odoriphaga Larvae
祝国栋 1丁文娟 2邱季雨 1王燕 2薛明 2赵海朋 2张国福3
作者信息
- 1. 聊城大学农学与农业工程学院,聊城 252000
- 2. 山东农业大学植物保护学院,泰安 271018
- 3. 山东省农药检定所,济南 250100
- 折叠
摘要
冻土毛霉菌株BO-1是新报道的韭菜迟眼蕈蚊病原真菌,但是关于其致病力和田间防效的针对性研究尚未见报道.本研究分析了冻土毛霉菌株BO-1对韭菜迟眼蕈蚊幼虫的致病力,探究了温度对菌株致病力的影响,并通过防效试验评价了其应用价值.结果表明,冻土毛霉菌株BO-1对韭菜迟眼蕈蚊幼虫致病活性较高,处理3 d对2龄和4龄幼虫的致死中浓度LC50分别为3.714×105和4.680×106孢子/mL;冻土毛霉菌株BO-1发挥高致病活性的适宜温度为16～28 ℃,尤以20～24 ℃最优.盆栽和田间试验证实,菌株BO-1对韭菜迟眼蕈蚊防效突出,并具有较好的持续控害作用.当孢子浓度为1×109孢子/mL时,处理后7d防效为95.57％,与化学杀虫剂噻虫胺(93.57％)相当.因此,将冻土毛霉菌株BO-1作为韭菜迟眼蕈蚊生防菌剂加以进一步开发可行性较强.
Abstract
Mucor hiemalis BO-1 is a newly reported entomopathogenic fungus that infects Bradysia odoriphaga,a destructive root maggot.However,the pathogenicity and field control efficiency of M.hiemalis BO-1 against B.odoriphaga are unknown.In this study,we analyzed the pathogenicity of M.hiemalis BO-1 to B.odoriphaga larvae and its relation to temperature and evaluated its application value by pot and field experiments.The results indicated that M.hiemalis BO-1 was of acute pathogenicity against B.odoriphaga larvae and was as toxic as some chemical pesticides.At 3 d after treatment,the median lethal concentrations(LC50)to the 2nd and 4th instar larvae were 3.714× 105 spores/mL and 4.680 × 106 spores/mL,respectively.Moreover,M.hiemalis BO-1 also showed pathogenic activities against B.difformis and Delia antiqua larvae.The optimal temperatures for high pathogenic activity of M.hiemalis BO-1 were 16-28 ℃,especially at 20-24 ℃.The results of pot and field experiments confirmed excellent control efficiency of M.hiemalis BO-1 against B.odoriphaga larvae,which exceeded 80％at 7 d after treatment with spore concentration of 1 X 107 spores/mL,the equivalence to the chemical insecticide clothianidin.In addition,M.hiemalis BO-1 exhibited high persistence in control of B.odoriphaga.Therefore,M.hiemalis BO-1 is of high perspective as a biocontrol agent against B.odoriphaga.
关键词
韭菜迟眼蕈蚊/冻土毛霉/昆虫病原真菌/致病力/防治效果Key words
Bradysia odoriphaga/Mucor hiemalis/entomopathogenic fungi/pathogenicity bioassay/control efficiency引用本文复制引用
基金项目
山东省自然科学基金(ZR201911130528)
聊城大学博士启动基金(318051815)
国家级大学生创新创业项目(CXCY2022042)
出版年
2024
国家科技期刊平台
NSTL
万方数据