Isolation and Identification of Arthrobacter pascens S17 and Its Ability to Promote Growth of Cucumber
In order to screen out the biocontrol bacteria with growth-promoting effect,soil samples were collected from Hebei,Shandong and other places,and the growth-promoting strains with high-yield IAA ability were isolated and screened,and the potential growth-promoting strains with growth-promoting ability were identified based on morphological characteristics,physiological and biochemical indexes and multi-gene phylogenetic tree analysis,and the growth-promoting effects of growth-promoting strains on cucumber seed germination and cucumber seedlings in pot experiments and field experiments were determined.The results showed that one bacterium,Arthrobacter pascens S17,which had a significant growth-promoting effect on cucumber seedlings,was isolated and identified.Arthrobacter pascens S17 could synthesize IAA in large quantities in DF+medium,and the highest IAA content was 128.54 μg/mL.The growth rate of cucumber seedling plant height and leaf area of cucumber seedlings reached 35.99％and 26.90％by root irrigation treatment with Arthrobacter pascens S17,and the physiological indexes of cucumber seedlings were significantly higher than those of the control.Arthrobacter pascens S17 has a significant effect on cucumber growth,and has a certain development and application prospect.
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