斜纹夜蛾高致病力生防真菌的筛选与鉴定
Screening and Identification of Biocontrol Fungus with High Pathogenicity against Spodoptera litura
田艺帆 1张正坤 2隋丽 2赵宇 2路杨 2孟钊 3石旺鹏 4李启云1
作者信息
- 1. 吉林省农业科学院植物保护研究所/农业农村部东北作物有害生物综合治理重点实验室/吉林省农业微生物重点实验室,长春 130033;中国农业大学植物保护学院,北京 100083
- 2. 吉林省农业科学院植物保护研究所/农业农村部东北作物有害生物综合治理重点实验室/吉林省农业微生物重点实验室,长春 130033
- 3. 公主岭市气象局,公主岭 136100
- 4. 中国农业大学植物保护学院,北京 100083
- 折叠
摘要
本研究从斜纹夜蛾幼虫僵虫中分离并纯化虫生真菌,得到 6株昆虫病原真菌,利用形态学和分子生物学方法对上述真菌进行鉴定,采用浸渍法测定 6 株真菌对斜纹夜蛾 2 龄幼虫的毒力.结果表明,6 株虫生真菌分别为2株球孢白僵菌Beauveria bassiana,以及莱氏绿僵菌Metarhizium rileyi,木贼镰刀菌Fusarium equiseti,曲霉菌Aspergillus nomiae和糙孢蓝状菌Talaromyces trachyspermus各 1株.在 1×108个孢子/mL浓度下,LT50分别为 4.737 d,4.543 d,1.343 d,2.086 d,2.212 d和 2.351 d,6株虫生真菌对斜纹夜蛾 2龄幼虫均表现出较强致病力,其中莱氏绿僵菌表现最好.本研究首次报道了镰刀菌和曲霉菌能够感染斜纹夜蛾,并首次发现糙孢蓝状菌能够感染昆虫.
Abstract
Six strains of entomogenous fungi were isolated and purified from muscardine cadavers of Spodoptera litura larvae,and were identified by morphological and molecular biological methods.The pathogenicity of these strains against S.litura larvae was determined by the dipping method.The six fungal strains were identified as Beauveria bassiana(two strains),Metarhizium rileyi,Fusarium equiseti,Aspergillus nomiae,and Talaromyces trachyspermus,whose LT50 against the pest larvae were 4.737,4.543,1.343,2.086,2.212,and 2.351 days,respectively.All the six strains of entomogenous fungi showed high pathogenicity against the pests at the concentration of 108 conidia/mL,in which M.rileyi established the highest pathogenicity.The present study reports for the first time that S.litura can be infected by F.equiseti and A.nomiae and that T.trachyspermus is able to infect insects.
关键词
斜纹夜蛾/虫生真菌/毒力Key words
Spodoptera litura/entomogenous fungi/pathogenicity引用本文复制引用
基金项目
吉林省科技厅中青年科技创新创业卓越人才团队项目(创新类)(20230508011RC)
出版年
2024
国家科技期刊平台
NSTL
万方数据