东北大黑鳃金龟幼虫高毒力球孢白僵菌菌株的筛选及应用
Screening and Application of High Toxicity Beauveria bassiana Strains against Holotrichia diomphalia Larvae
张强 1张云月 1朱晓敏 1路杨 1常雪 1高悦 2赫思聪 1李茂海 1田志来1
作者信息
- 1. 吉林省农业科学院植物保护研究所/吉林省农业微生物重点实验室/农业农村部东北作物有害生物综合治理重点实验室,公主岭 136100
- 2. 吉林省吉兴农业技术服务中心,公主岭 136100
- 折叠
摘要
为了筛选到针对靶标害虫东北大黑鳃金龟幼虫高毒力球孢白僵菌菌株,通过室内生物测定,明确球孢白僵菌BbQCGZL-1 对东北大黑鳃金龟幼虫的致病力,并与其他药剂进行防效对比;同时通过盆栽试验测定了球孢白僵菌 BbQCGZL-1 在不同农作物上对东北大黑鳃金龟幼虫的防治效果.球孢白僵菌BbQCGZL-1 在施药 14 d时校正死亡率达到 75%以上,高于其他供试菌株;施药 7 d时LC50为 1.21×107 cfu/mL,当菌悬液浓度为 1.0×108 cfu/mL时,LT50为 8.82 d;室内生测试验和室外盆栽试验结果表明,在东北地区利用球孢白僵菌防治东北大黑鳃金龟幼虫时,球孢白僵菌BbQCGZL-1 与商品化的微生物源药剂在防治效果上无显著差异.球孢白僵菌BbQCGZL-1 可作为防治东北大黑鳃金龟幼虫的有效微生物源药剂.
Abstract
The aim of this study was to screen for highly toxicity Beauveria bassianan strains against the larvae of Holotrichia diomphalia Bates.The pathogenicity of a B.bassiana strain BbQCGZL-1 against the white grubs was determined by laboratory bioassay and its control efficiency against the grubs was compared to other agents.Further,the control efficiency of B.bassiana BbQCGZL-1 against the white grubs on different crops was tested by using outdoor potted plants.The results showed that the corrected mortality rate of the grubs was more than 75%at 14 d post treatment with the B.bassiana BbQCGZL-1,higher than those obtained with other stains tested;The LC50 was 1.21×107 cfu/mL at 7 d post treatment,the LT50 was 8.82 d with a concentration of spore suspension of 1.0×108 cfu/mL.The results of the laboratory bioassay and the outdoor potted plant experiment showed no significant difference in the control efficiency between B.bassiana BbQCGZL-1 and commercial biological agents against the white grubs in Northeast of China.Overall,the B.bassiana BbQCGZL-1 can be used as a microbial agent to control H.diomphalia larvae in the field.
关键词
东北大黑鳃金龟/蛴螬/球孢白僵菌Key words
Holotrichia diomphalia/grubs/Beauveria bassiana引用本文复制引用
基金项目
吉林省农业科技创新工程项目(CXGC2021ZY021)
吉林省科技厅重点研发项目(20210202122NC)
吉林省科技厅重点研发项目(20210202092NC)
出版年
2024
国家科技期刊平台
NSTL
万方数据