60Co-NTG复合诱变选育丁烯基多杀菌素高产菌株及其杀虫活性
Breeding of Butenyl-spinosyns High Yielding Strain by 60Co-NTG Compound Mutation and Its Insecticidal Activity
徐周钦 1郭超 2李金萍 3张逍遥 2王珂慧 2何志平 4刘兴泉 4王超2
作者信息
- 1. 浙江农林大学食品与健康学院,杭州 311300;国家粮食和物资储备局科学研究院粮油加工研究所,北京 100037
- 2. 国家粮食和物资储备局科学研究院粮油加工研究所,北京 100037
- 3. 北京市植物保护站,北京 100029
- 4. 浙江农林大学食品与健康学院,杭州 311300
- 折叠
摘要
为获得稳定高产的丁烯基多杀菌素生产菌株并明确其对农业害虫的杀灭效果,以前期研究获得的须糖多孢菌Saccharopolyspora pogona ASAGF19 为初始菌株,通过 60Co-γ射线和亚硝基胍复合诱变得到 1株遗传性状稳定的高产突变株 ASAGF30A11,较初始菌株产量提高了 61.26%,3 次传代产量变化幅度小于10.00%.对高产菌株发酵液进行分离纯化获得了丁烯基多杀菌素粗品,配制成 1.5 g/L浓度的母液进行稀释,对蓟马、蚜虫以及小菜蛾进行室内毒力测定,得出其对3种供试害虫的LC50分别为1.92 mg/L、1.23 mg/L和 0.27 mg/L.经过比较,丁烯基多杀菌素对蓟马的杀灭效果优于多杀菌素(LC50为 0.57 mg/L)和乙基多杀菌素(LC50为 0.64 mg/L);对蚜虫的杀灭效果优于多杀菌素(LC50为 72.60 mg/L),低于阿维菌素(LC50为 0.14 mg/L);对小菜蛾的杀灭效果优于多杀菌素(LC50为2.87 mg/L),低于乙基多杀菌素(LC50为0.05 mg/L).本研究首次探索了 60Co-γ射线和亚硝基胍复合诱变对须糖多孢菌的影响,并获得了丁烯基多杀菌素对 3种农业害虫的防效,为新型生物杀虫剂对农业害虫的防治提供了一些理论依据.
Abstract
In order to obtain stable and high-yield butenyl-spinosyn-producing strains and determine its lethal efficiency against agricultural pests,a high-yield mutant strain ASAGF30A11 with stable genetic traits was obtained by compound mutagenesis of a parent strain Saccharopolyspora pogona ASAGF19 screened from the previous study using 60Co-γ-ray and nitrosoguanidine.The new strain increased yield by 61.26%compared with the parent strain,and the yield variation of 3 generations was less than 10%.The crude butenyl-spinosyn was purified from the fermentation broth of the high-yield strain,and then diluted into the mother liquor with a concentration of 1.5 g/L.The insecticidal activity against Thrips palmi Karny,aphids and Plutella xylostella was investigated and the LC50 was determined to be 1.92 mg/L,1.23 mg/L and 0.27 mg/L,respectively.By comparison,butenyl-spinosyn was of a higher lethal efficiency against T.palmi than spinosyn(LC500.58 mg/L)and spinetoram(LC500.64 mg/L),a higher lethal efficiency against aphids than spinosyn(LC5072.60 mg/L)but equal efficiency with avermectin(LC500.14 mg/L),and a higher lethal efficiency against P.xylostella than spinosyn(LC502.9 mg/L)but lower efficiency than spinetoram(LC500.05 mg/L).This study explored the effect of compound mutagenesis using 60Co-γ-ray and nitrosoguanidine on S.pogona for the first time and measured the laboratory lethal efficiency of butenyl-spinosyn against three agricultural pests,which serves as part of the theoretical basis for the control of agricultural pests with new biological insecticides.
关键词
须糖多孢菌/60Co-γ射线/亚硝基胍/复合诱变/丁烯基多杀菌素Key words
Saccharopolyspora pogona/60Co-γ-ray/NTG/combined mutagenesis/butenyl-spinosyns引用本文复制引用
基金项目
国家重点研发计划(2023YFA0914700)
中央级公益性科研院所基本科研业务费专项(JY2311)
企业合作技术开发项目(H20054-1)
出版年
2024
国家科技期刊平台
NSTL
万方数据