Isolation,Identification and Evaluation of Insecticidal Activity of an Entomopathogenic Bacteria Strain Infecting Post-overwintering Ostrinia furnacalis Larvae
A bacterial strain ACB3014 was isolated from the infected overwintering larvae of Ostrinia furnacalis in spring sown corn fields in Xinyuan County,Yili Valley,Xinjiang and identified by morphological observation,physiological and biochemical characteristics and 16S rDNA sequence analysis.The pathogenicity of this strain against the 3rd instar larvae of O.furnacalis was determined by feeding method.The strain ACB3014 was Serratia marcescens.The cumulative corrected mortality of ACB3014 on the 3rd instar larvae increased with the increase of bacterial suspension concentration.On the 7th day after feeding ACB3014 bacterial suspension,the cumulative adjusted mortality of the 3rd instar larvae ranged between 27.78%—77.78%at the concentration of 2×104-2×108 cfu/mL.On the 13th day of inoculation,when the concentration reached 2×108 cfu/mL,LC50 and LT50 against the 3rd instar larvae of O.furnacalis were 3.79×103 cfu/mL and 3.93 days,respectively.In addition,the infected O.furnacalis larvae are dark red and black and are pyoid and festering,giving off a strong odor.In conclusion,ACB3014 strain has good insecticidal activity against O.furnacalis larvae and has a high potential as a green biological control resource for O.furnacalis.
Ostrinia furnacalisSerratia marcescensdetermination of virulencepathogenicinsecticidal activity
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