Functional Verification of Endophytic Bacillus subtilis WZ10 and Its Control Efficacy on Potato Fusarium Wilt
To clarify the biological function of Bacillus subtilis WZ10 and its indoor control efficiency on potato Fusarium wilt,growth promoting ability of strain WZ10 was tested by Salkowski's colorimetric and spectrophotometry methods and pot experiment.Biocontrol effect was determined by pot experiment and physiology and biochemistry measure,and phosphorus and kalium degradation abilities were tested by transparent zone method.The preventive effect of strain WZ10 was analyzed by laboratory test and physiological and biochemical determination.The results showed that WZ10 could produce IAA and degrade inorganic phosphorus,and showed significant control efficiency on potato Fusarium wilt.Compared to the control,the plant height,root length and fresh weight of plants were significantly increased by 14.34%,19.75%and 77.07%,respectively and the activities of PPO,POD,SOD and soluble protein amount of plants were significantly increased after strain WZ10 inoculation.Meanwhile,the control efficacy of strain WZ10 on potato Fusarium wilt was 80.65%,significantly higher than that of 30%metriaxalin treatment.In this study,strain WZ10 had strong growth promotion ability and significant control effect on potato Fusarium wilt,which will lay a foundation for the subsequent functional development and potato Fusarium wilt control.
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