拮抗放线菌2-1-2F的鉴定及其对黄瓜枯萎病的防效
Identification of Antagonistic Actinomycetes 2-1-2F and Its Control Effect on Cucumber Fusarium Wilt
蒋冬阳 1吴冰越 1季文平 1陈宸 1陈夕军1
作者信息
- 1. 扬州大学植物保护学院,扬州 225009
- 折叠
摘要
为获得可高效防控黄瓜枯萎病的生防放线菌,采用梯度稀释和平板对峙法从蔬菜根际土壤中筛选到1株对黄瓜枯萎病菌Fusarium oxysporum f.sp.cucumerinm(FOC)有较强拮抗作用的放线菌 2-1-2F;经形态学和分子生物学鉴定,确定菌株 2-1-2F为弗吉尼亚链霉菌Streptomyces virginiae.其能产生蛋白酶、β-葡聚糖酶和几丁质酶,不产生嗜铁素和纤维素酶,且菌株 2-1-2F产生的发酵滤液活性易受环境条件影响.菌株 2-1-2F发酵菌液对黄瓜有明显的促生作用,可诱导黄瓜体内防御酶活性和抗性相关物质含量增加,并于处理后 1～7 d内达最大值.菌株 2-1-2F在黄瓜根部有较强的定殖能力,42 d后在土壤中仍可达 106 cfu/g;其对黄瓜枯萎病的预防和治疗效果分别达 48.98%和 34.01%.
Abstract
In order to obtain biocontrol actinomycetes to control cucumber Fusarium wilt,a strain 2-1-2F with strong antagonistic activity against F.oxysporum f.sp.cucumerinum(FOC)was isolated from vegetable rhizospheric soil by gradient dilution and confrontation culture methods.Strain 2-1-2F was identified as Streptomyces virginiae by morphology observation and molecular biology identification.It could produce proteases,chitinases and β-glucanases,but not produce siderophores and cellulases.The activity of fermentation liquid produced by 2-1-2F was susceptible to environmental conditions.Fermentation liquid of 2-1-2F had obvious growth-promoting effect on cucumber plants,and could induce the cucumber defense enzyme activities and resistance related substance contents,which reached the maximum value from one to seven days after treatment.Strain 2-1-2F had strong colonization ability in cucumber roots,and the concentration of colonization could still reach 106 cfu/g soil after 42 days.The preventive and therapeutic effects of 2-1-2F on cucumber Fusarium wilt were 48.98%and 34.01%,respectively.Actinomycetes 2-1-2F and its metabolites had the potential to be developed into biocontrol agents.
关键词
弗吉尼亚链霉菌/鉴定/黄瓜枯萎病/生防效果Key words
Streptomyces virginiae/identification/cucumber Fusarium wilt/control effect引用本文复制引用
基金项目
现代农业产业技术体系建设项目(CARS-24-C-01)
出版年
2024
国家科技期刊平台
NSTL
万方数据