Optimization of Solid-State Fermentation Process of Trichoderma citrinoviride GF-11 on Rosa roxburghii Tratt Pomace by Response Surface Methodology
Trichoderma spp.exhibit the capability to inhibit pathogen,promote plant growth,and enrich soil nutrition.The pomace derived from chestnut rose(Rose roxburghii Tratt.),a byproduct of chestnut rose juice processing,holds significant potential for valuable reutilization.This study aims to employ chestnut rose pomace as the primary substrate and wheat bran as the supplementary material for solid fermentation.The conidial quantity of Trichoderma citrinoviride GF-11 during solid-state fermentation was used as the screening criterion,with optimization of the fermentation conditions through a Plackett-Burman experimental design and a Box-Behnken design-response surface method.The results indicated that the proportion of bran,inoculum quantity,and fermentation duration are the key factors in the culture medium formula,exerting a significant impact on the sporulation of Trichoderma citrinoviride GF-11.The optimized fermentation culture conditions include wheat bran(61%),inoculation amount(10.48%),zinc sulfate(0.5%),moisture content(250%),and fermentation time(10 d).Under these specified conditions,the fermentation of Trichoderma citrinoviride GF-11 yielded 2.32×1010 CFU/g of conidia.This study not only provides suitable process conditions for the fermentation of chestnut rose pomace,and a pathway to decrease environmental pollution,but also enhance the efficient utilization of the pomace.Furthermore,it introduces a new approach for producing high-quality bio-organic fertilizer.
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