首页|抗烟草PVY生防细菌D3-1的筛选鉴定及活性物质分析

抗烟草PVY生防细菌D3-1的筛选鉴定及活性物质分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
由马铃薯Y病毒(Potato virus Y,PVY)引起的病毒病在烟草种植地区大面积发生,对烟草品质和产量产生重大影响.采用半叶枯斑法筛选出对烟草PVY防治效果优良的生防细菌D3-1,对PVY的枯斑抑制率达 99.71%,经形态学、生理生化特性以及 16S rDNA 序列鉴定其为解淀粉芽胞杆菌 Bacillus amyloliquefaciens.菌株D3-1 具有广谱的抑菌能力,可抑制病原真菌和细菌生长,菌株D3-1发酵液(1×109 CFU/mL)对烟草PVY的田间防治效果为 63.67%.全基因组测序分析发现菌株D3-1 基因组含有表面活性素surfactin、丰原素fengycin、嗜铁素bacillibactin等 12种具有抑菌作用的次生代谢产物合成基因簇,经HPLC-MS定性分析确定了表面活性素surfactin和丰原素fengycin等 32个脂肽类提取物.菌株D3-1 对PVY有良好防治效果,具有作为防治烟草PVY生防菌剂的潜力.
Screening,Identification and Active Substance Analysis of Biocontrol Bacterium D3-1 against Tobacco PVY
Viral diseases caused by potato virus Y(PVY)occur extensively in tobacco planting areas,significantly impacting tobacco quality and yield.Through half-leaf necrosis spot method,the biocontrol bacterium D3-1 with excellent efficacy against PVY in tobacco was screened,exhibiting an inhibition rate of 99.71%.Based on morphological,physiological,biochemical characteristics and 16S rDNA sequence analysis,D3-1 was identified to be Bacillus amyloliquefaciens.D3-1 strain displayed broad-spectrum anti antimicrobial activity,inhibiting the growth of pathogenic fungi and bacteria.Field trials with D3-1 fermentation broth(1×109 CFU/mL)resulted in an efficacy of 63.67%against PVY in tobacco.Whole-genome sequencing revealed that strain D3-1 harbored 12 gene clusters responsible for the synthesis of secondary metabolites,including surfactin,fengycin,and bacillibactin,known for their antimicrobial properties.Qualitative analysis using HPLC-MS confirmed the presence of 32 peptide extracts,including surfactin and fengycin.Strain D3-1 exhibited promising efficacy against PVY,demonstrating its potential as a biocontrol agent for managing PVY in tobacco.

PVYBacillus amyloliquefacienswhole-genome sequencinggene cluster of secondary metabolite synthesisactive substance

李玲玲、陈奕冰、滕凯、肖志鹏、周向平、肖艳松、蔡海林、董鹏、梅斯钇、刘索妮、伍绍龙、刘天波、唐前君

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湖南农业大学植物保护学院,长沙 410128

中国烟草总公司湖南省公司,长沙 410004

PVY Bacillus amyloliquefaciens 全基因组测序 次生代谢产物合成基因簇 活性物质

湖南省重点领域研发计划中国烟草总公司项目中国烟草总公司项目湖南省烟草公司衡阳市公司项目

2023NK2019110202101050LS-10110202201019LS-03HYYC2023KJ32

2024

10.16409/j.cnki.2095-039x.2024.02.020

中国生物防治学报
中国农业科学院植物保护研究所 中国植物保护学会

中国生物防治学报

CSTPCD北大核心
影响因子：1.054
ISSN：2095-039X
年,卷(期)：2024.40(3)
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