抗烟草PVY生防细菌D3-1的筛选鉴定及活性物质分析
Screening,Identification and Active Substance Analysis of Biocontrol Bacterium D3-1 against Tobacco PVY
李玲玲 1陈奕冰 1滕凯 2肖志鹏 2周向平 2肖艳松 2蔡海林 2董鹏 1梅斯钇 1刘索妮 1伍绍龙 2刘天波 2唐前君1
作者信息
- 1. 湖南农业大学植物保护学院,长沙 410128
- 2. 中国烟草总公司湖南省公司,长沙 410004
- 折叠
摘要
由马铃薯Y病毒(Potato virus Y,PVY)引起的病毒病在烟草种植地区大面积发生,对烟草品质和产量产生重大影响.采用半叶枯斑法筛选出对烟草PVY防治效果优良的生防细菌D3-1,对PVY的枯斑抑制率达 99.71%,经形态学、生理生化特性以及 16S rDNA 序列鉴定其为解淀粉芽胞杆菌 Bacillus amyloliquefaciens.菌株D3-1 具有广谱的抑菌能力,可抑制病原真菌和细菌生长,菌株D3-1发酵液(1×109 CFU/mL)对烟草PVY的田间防治效果为 63.67%.全基因组测序分析发现菌株D3-1 基因组含有表面活性素surfactin、丰原素fengycin、嗜铁素bacillibactin等 12种具有抑菌作用的次生代谢产物合成基因簇,经HPLC-MS定性分析确定了表面活性素surfactin和丰原素fengycin等 32个脂肽类提取物.菌株D3-1 对PVY有良好防治效果,具有作为防治烟草PVY生防菌剂的潜力.
Abstract
Viral diseases caused by potato virus Y(PVY)occur extensively in tobacco planting areas,significantly impacting tobacco quality and yield.Through half-leaf necrosis spot method,the biocontrol bacterium D3-1 with excellent efficacy against PVY in tobacco was screened,exhibiting an inhibition rate of 99.71%.Based on morphological,physiological,biochemical characteristics and 16S rDNA sequence analysis,D3-1 was identified to be Bacillus amyloliquefaciens.D3-1 strain displayed broad-spectrum anti antimicrobial activity,inhibiting the growth of pathogenic fungi and bacteria.Field trials with D3-1 fermentation broth(1×109 CFU/mL)resulted in an efficacy of 63.67%against PVY in tobacco.Whole-genome sequencing revealed that strain D3-1 harbored 12 gene clusters responsible for the synthesis of secondary metabolites,including surfactin,fengycin,and bacillibactin,known for their antimicrobial properties.Qualitative analysis using HPLC-MS confirmed the presence of 32 peptide extracts,including surfactin and fengycin.Strain D3-1 exhibited promising efficacy against PVY,demonstrating its potential as a biocontrol agent for managing PVY in tobacco.
关键词
PVY/Bacillus/amyloliquefaciens/全基因组测序/次生代谢产物合成基因簇/活性物质Key words
PVY/Bacillus amyloliquefaciens/whole-genome sequencing/gene cluster of secondary metabolite synthesis/active substance引用本文复制引用
基金项目
湖南省重点领域研发计划(2023NK2019)
中国烟草总公司项目(110202101050LS-10)
中国烟草总公司项目(110202201019LS-03)
湖南省烟草公司衡阳市公司项目(HYYC2023KJ32)
出版年
2024
国家科技期刊平台
NSTL
万方数据