Identification of Pathogen Causing Bacterial Shot Hole of Peaches in Pinggu District of Beijing and Screening of Its Antagonistic Microorganisms
To obtain strains that inhibit bacterial perforation of peach in Pinggu District,we screened antagonistic bacteria after identifying the pathogen through DNA sequencing.The peach perforating pathogen was isolated from morbid peach fruits in Pinggu District using a tissue isolation method and pathogenicity was determined.Based on morphological characteristics and 16S rDNA sequence similarity analysis,the pathogen was preliminarily identified as Xanthomonas arboricola pv.pruni.Five bacterial strains had antagonistic activity using the plate standoff method.Among them,strain xj39 exhibited the most significant inhibitory effect,with a diameter of the inhibition zone reaching 54.7 mm.The second was strain xj12,with a diameter of the inhibition zone of 50.8 mm.16S rDNA and rpoB gene sequences analysis indicated that strain xj39 was Streptomyces fimbriatus and strain xj12 was Streptomyces ambofaciens.Field prevention test showed that the fermentation solution of xj39 had the control efficacy of 33%against peach bacterial perforation disease,which was not significantly different from that of the chemical kasugamycin treatment.
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