Control Effect of Bacillus methylotrophicus NKG-1 on Tomato Gray and Its Genome-Wide Analysis
To investigate control effect of Bacillus methylotrophicus NKG-1 on tomato gray mold,dry weight of B.cinerea and inhibition rate and control efficacy of NKG-1 on tomato gray mold were investigated after treatment with the fermentation broth of NKG-1.The results showed that NKG-1 significantly inhibited the hyphae growth and spore production of B.cinerea.The control efficacy of NKG-1 against gray mold on tomato leaves in vitro was 80.00%,and that on tomato plant at seedling stage was 73.14%.By analyzing and predicting the whole genome of NKG-1,it was found that the NKG-1contained 12 secondary metabolite gene clusters.Among them,lipopeptide antibiotics surfactant and fengycin have antibacterial effects.Fengycin has been proved to have strong antifungal activity.The above results provide an important theoretical basis for application of B.methylotrophicus NKG-1 to control tomato gray mold and are helpful to analyze the biological control mechanism of B.methylotrophicus NKG-1.
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