Control Effect of Streptomyces roseoflavus and Fungicides on Phytophthora nicotianae
This study aims to improve control effect of Streptomyces roseoflavus LN33 on tobacco black shank by screening out fungicides and ratio combined with LN33.Toxicity of 3 kinds of fungicides and LN33 on Phytophthora nicotianane was determined with inhibition zone method and EC50 values were calculated.Plate culture count method was used to determine the biocompatibility of fungicides and LN33,and the mixture scheme was determined with Horsfall method.The results showed that the EC50 values of metalaxyl and dimethomorph against P.nicotianane were 0.26 and 0.92 μg/mL,respectively,while the EC50 value of metalaxyl was the lowest,which was only 0.26 μg/mL,and metalaxyl showed good biocompatibility with LN33.The IR value was 1.032 when volume ratio was 6:4 of 0.26 μg/mL metalaxyl and 3.41×107 cfu/mL LN33.The growth inhibition rate and control efficacy of compounds on P.nicotianae in pot experiment were 86.96%and 77.06%,respectively,significantly higher that single dose.Therefore,metalaxyl can combine with S.roseoflavus LN33 to control P.nicotianae wilt,and only half amount of fungicide would be applied in the compound.
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