十字花科根肿病拮抗菌贝莱斯芽胞杆菌ZF72分离鉴定及生防效果研究
Identification and Biocontrol Effect of Bacillus velezensis ZF72 against Clubroot disease of Crucifers
林子涵 1张思雨 2康华军 2袁晓伟 3李兴盛 3石延霞 2谢学文 2李磊 2范腾飞 2李宝聚 2柴阿丽2
作者信息
- 1. 中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京 100081;中国农业大学园艺学院,北京 100193
- 2. 中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京 100081
- 3. 山东省华盛农业集团股份有限公司,青州 262500
- 折叠
摘要
针对十字花科根肿病日益严重且防治困难的问题,采用平板对峙法,以尖孢镰孢菌Fusarium oxysporum和辣椒疫霉菌Phytophthora capsici为指示菌进行初筛,通过盆栽试验复筛,得到十字花科根肿病高效生防菌株ZF72,盆栽防效 74.6%.根据形态学特征、生理生化特性及 16S rDNA,gyr B和atp D多基因系统发育分析,鉴定菌株ZF72 为贝莱斯芽胞杆菌Bacillus velezensis.采用单因素试验对菌株ZF72 摇瓶发酵工艺进行优化,确定最佳配方为山梨醇 3 g/L、麸皮 8 g/L、CaCl2 1 g/L和MgCl2·6H2O 3 g/L,最适发酵条件为温度 28℃、pH 6.0、装液量 50 mL/250 mL、转速 240 r/min、种子液接种量 1%(v/v).田间试验结果显示,菌株ZF72 处理根肿病症状明显减轻,田间防效可达 63.8%,为根肿病生物防治提供了新的微生物资源.
Abstract
Clubroot disease of cruciferous plants caused by Plasmodiophora brassicae Woronin occurred severely and was difficult to control.In this study,a biocontrol agent ZF72 was screened by using dual-culture assay against Fusarium oxysporum and Phytophthora capsica.Control efficacy of ZF72 against clubroot disease in pot experiment was 74.6%.The strain ZF72 was identified as Bacillus velezensis based on morphological characteristics,physiological and biochemical analysis,as well as 16S rDNA,gyrB and atpD phylogenetic analysis.The fermentation of strain ZF72 was optimized by single factor test.An optimum medium composition was as followings:sorbitol 3 g/L,bran 8 g/L,CaCl2 1 g/L,and MgCl2·6H2O 3 g/L.The optimum fermentation conditions were as followings:temperature 28℃,pH 6.0,loading volume 50 mL/250 mL,rotating speed 180 r/min,and inoculation amount 1%(v/v).Treatment with strain ZF72 significantly reduced the incidence of clubroot disease of Chinese cabbage,with a control efficacy of 63.8%in field experiment,which provides new microbial resources for biological control of clubroot disease of crucifers.
关键词
十字花科根肿病/贝莱斯芽孢杆菌/生物防治Key words
clubroot of crucifers/Bacillus velezensis/biocontrol引用本文复制引用
基金项目
国家重点研发计划(2022YFD1401200)
中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS)
大宗蔬菜产业技术体系(CARS-25)
出版年
2024
国家科技期刊平台
NSTL
万方数据