Study on Mixture of Metarhizium robertsii with Matrine to Control Aphis gossypii
Aphis gossypii is an important piercing-sucking pest with broad spectra of hosts.In order to develop biocontrol technology for this pest,this research was focus on the mixture of entomopathogenic fungal conidia and matrine.The bioactivity of the insecticides against A.gossypii was tested using spray and the biocompatibility of matrine to fungi was determined by colony growth and spore germination.Meanwhile,the cotoxicity factor(CF)was used to evaluate the joint bioactivity of fungi and matrine,and pot-test was employed to assess the effect of the mixed preparation to aphid.The results of laboratory bioassay showed that the strains of Metarhizium robertsii MrCb01 and MrCb02,M.anisopliae MaGX19S02,and Cordyceps cateniannulata IcCb01 had high bioactivities against A.gossypii,which caused＞80%mortality rate of A.gossypii at the fungal concentration of 1×108 spores/mL at 72 h post-treatment.The results of biocompatibility test showed that matrine exerted no significant effects on mycelial growth and spore germination of MrCb01 strain.Furthermore,a synergism of MrCb01 conidia and matrine at 20:1(m:m)was found,as indicated by a CF value of 22.45.Finally,a wettable powder mixure of MrCb01 conidia and matrine was prepared,which achieved an 85.41%control efficacy of aphid at 7 d post-treatment by 200 times dilution in pot-cucumber test.Our experiment provides evidence that the mixture of MrCb01 and matrine has practical potential in controlling aphids.
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