The CsInR Gene Regulates Lipid Accumulation in the Diapause Seven-spotted Ladybeetle
The seven-spotted ladybeetle,Coccinella septempunctata L.,is a predatory natural enemy widely used as a biological control agent against pests.The diapause of C.septempunctata is induced by low temperature and short light.The diapause females accumulate a large amount of lipid in the abdomen and display a prolonged longevity.The technology of diapause induction is expected to extend the shelf-life of natural enemy insect products and improve the application of the products in biological control programs.Insulin signaling plays an essential role in the physiological changes of insects.Its function on the diapause process of C.septempunctata remains unclear.We identified and analyzed the insulin receptor gene(CsInR)sequence of C.septempunctata.The RT-qPCR results showed that,compared with that in the nondiapause individuals,the CsInR expression was significantly downregulated in the diapause individuals'heads.The CsInR gene expression of the individuals with diapausing induction for 20 days was significantly lower than that of those individuals induced for diapause for other periods.After RNA interference with the CsInR gene,the lipid content and the expression of essential genes of the lipid synthesis pathway(CsFas1,CsFas2 and CsFad△11 genes)were significantly reduced.This study paves the way for further investigating the mechanism of insulin signaling regulating lipid accumulation during diapause in C.septempunctata..
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