抗生素溶杆菌中PAS-LuxR家族转录因子正向调控吩嗪类抗菌物质myxin的生物合成
PAS-LuxR Family Transcription Factor Positively Regulates the Biosynthesis of Antimicrobial Phenazine Myxin in Lysobacter antibioticus
赵杨扬 1陈俊菁 1徐高歌 2承心怡 2刘凤权3
作者信息
- 1. 江苏省农业科学院植物保护研究所,南京 210014;海南大学热带农林学院,海口 570228
- 2. 江苏省农业科学院植物保护研究所,南京 210014
- 3. 江苏省农业科学院植物保护研究所,南京 210014;贵州大学农学院,贵阳 550025
- 折叠
摘要
溶杆菌 Lysobacter 是一类具有生防潜力的革兰氏阴性细菌.氮氧化吩嗪 myxin 是从抗生素溶杆菌Lysobacter antibioticus OH13中分离鉴定到的一种对病原细菌、真菌、卵菌等均具有较强拮抗活性的抗菌物质.我们已对myxin的生物合成机制进行了解析,但其生物合成的调控因子还鲜有报道.本研究在myxin生物合成基因簇上游鉴定到 PAS-LuxR 家族调控因子编码基因 LaPhzR,LaPhzR 敲除突变体丧失了产生myxin 等吩嗪物质的能力,也丧失了对水稻条斑病菌 RS105 的拮抗能力,且 myxin 合成基因与解毒基因LaPhzX均不能表达.这些结果表明LaPhzR正向调控myxin的生物合成,且对myxin的生物合成是必需的.当在野生型菌株中过表达LaPhzR,myxin产量可提高 1.6倍,这对myxin的开发应用具有重要意义.
Abstract
Lysobacter,a genus of Gram-negative bacteria,is emerging as a novel source of biocontrol agents against phytopathogenic microorganisms.Previously,we isolated an N-oxide phenazine myxin from Lysobacter antibioticus OH13,demonstrating potent activity against bacteria,fungi,and oomycetes.Furthermore,we elucidated the biosynthetic pathway of myxin,however,there are few reports concerning its regulatory mechanisms.In this study,we identified a PAS-LuxR family transcriptional regulator encoding gene,LaPhzR,located upstream of the myxin biosynthetic gene cluster.Deletion of LaPhzR resulted in deficiency of myxin production and antagonism against Xanthomonas oryzae pv.oryzicola(Xoc)RS105.Additionally,the LaPhzR mutant exhibited impaired expression of myxin biosynthetic genes and detoxification gene LaPhzX.These findings indicate that LaPhzR positively regulates myxin biosynthesis,a crucial process for myxin production.Overexpression of LaPhzR in the wild type strain led to 1.6-fold increase in myxin production,highlighting its significance in myxin development.
关键词
溶杆菌/吩嗪/myxin/转录调控因子/PAS-LuxRKey words
Lysobacter/phenazine/myxin/transcriptional regulator/PAS-LuxR引用本文复制引用
基金项目
国家自然科学基金(32172492)
国家自然科学基金(31701840)
出版年
2024
国家科技期刊平台
NSTL
万方数据