Abstract
Objective:To investigate the effect and mechanism of bioactive glass 1393 on wound healing in mice with burn injuries.Methods:A benchtop tempera-ture-controlled scald instrument was used to establish a mouse burn wound model,which was divided into an injury group(simple scald group)and a bioactive glass 1393 treatment group.The wound healing of burn injuries in mice was observed at different times after the operation,and the formation of granulation tissue,collagen deposition and tissue proliferation in the wounds were observed by HE staining,Masson staining and Ki67 staining,and the levels of apoptosis-related proteins and the expression of wnt3a/β-catenin signaling pathway were detected in the wounds by Western blot.Re-sults:Compared with the injury group,the bioactive glass 1393 group accelerated burn wound healing and promoted granulation tissue formation,collagen deposition,and cell proliferation.In addition,compared with the injury group,the bioactive glass 1393 group promoted the expression of Bcl-2 protein,inhibited the expression of Bax protein and up-regulated the expression of Wnt3a and β-catenin protein.Conclusion:Bioactive glass 1393 promotes burn wound healing in mice by inhibiting apoptosis through modu-lation of wnt3a/β-catenin signaling pathway.
基金项目
Zhejiang Natural Science Foundation Project(LQ23H110002)
NETL
NSTL
万方数据