Abstract
Objective:To construct methotrexate(MTX)-chitosan(CS)-chuanxiong-muizine(TMP)nano-delivery system MTX-CS-TMP-NPs,and to investigate its effect on the telomerase activity of cervical cancer Hela cells.Methods:MTX-CS and MTX-CS-TMP-NPs were prepared by amide reaction and ionic gel method,and the encapsulation rate and drug loading rate were observed.MTT method and TRAP method were used to detect Hela cells and telomerase activity,and to compare the difference of telomerase activity of MTX,MTX-CS,and MTX-CS-TMP-NPs on cervical cancer Hela cells.Results:In this study,the total amount of TMP was 150 μg,of which the amount of free TMP was(26.46±1.72)μg,the encapsulation rate of TMP was(82.36±0.01)%,and the drug loading was(17.62±0.23)mg.The activity of Hela cells increased with the extension of time,and the OD value of Hela cell activity(1.24±0.05)was lower than that of the other three groups at 72 h of treatment,and the difference was statistically significant(P<0.05).The telomerase activity of HeLa cells in the MTX-CS-TMP-NPs solution treatment group was(0.23±0.12)%lower than that of the other three groups,with statistically significant difference(P<0.05).Conclusion:MTX-CS-TMP-NPs may effectively reduce the activity and telomerase activity of cervical cancer Hela cells,showing its potential anti-cervical cancer effect.
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