Abstract
Objective:To investigate the effect of miR-197 regulation on the invasion and migration of uterine fibroid cells,and to analyze the relationship between miR-197 and the RON/PI3K signaling pathway. Methods:A total of 48 magnetic SD rats were selected,and were divided into four groups after adaptive feeding,with 12 rats in each group. Except for the blank group,the other three groups of rats were intramuscularly injected with 0.05 mg/(100 g·d) estradiol benzoate and 0.5 mg/(100 g·d) progesterone to construct a rat model of uterine fibroids. After successful modeling,the rats in the blank group and the model group were given 200 mg/(kg·d) normal saline by gavage,the rats in the up-regulation group were given 10 nmol/(L·d) pcDNA3.1-miR-197 by gavage,and the rats in the down-regulation group were given 10 nmol/(L·d) miR-197-siRNA by gavage. After continuous treatment for 7 days,the expression levels of miR-197,the numbers of invaded and migrated uterine fibroids cells,and the expression levels of proteins related to the RON/PI3K signaling pathway in the four groups of rats were observed and compared. Results:There were statistically significant differences in the expression levels of miR-197 among the four groups (P<0.05),and the expression level of miR-197 in the up-regulation group was higher than that in the other three groups (P<0.05). There were statistically significant differences in the numbers of invaded and migrated cells among the four groups (P<0.05),the numbers of invaded and migrated cells in the up-regulation group were higher than those in the blank group and lower than those in the model group and the down-regulation group (P<0.05). There were statistically significant differences in the expression levels of RON and PI3K among the four groups (P<0.05),the expression level of RON in the up-regulation group was higher than that in the blank group and lower than that in the model group and the down-regulation group,and the expression level of PI3K was higher than that in the down-regulation group (P<0.05). Conclusion:Up-regulation of miR-197 can inhibit the invasion and migration of uterine fibroid cells in model rats,and the mechanism may be related to the RON/PI3K signaling pathway.
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