Effects of Isoflurane Anesthesia on Spontaneous and TUS/TMAS Induced Electromyography in Mice
Transcranial ultrasound stimulation(TUS)and transcranial magneto-acoustic stimulation(TMAS)are effective in modulating motor cortex.Previous studies have mostly been conducted under anesthesia confined to the difficulty of tethering conscious animals,and the analysis of the attenuating modulatory effects of anesthesia has primarily focused on the central nervous system.In this study,spontaneous electromyography(EMG)and TUS/TMAS-induced EMG were recorded during isoflurane anesthesia in 24 mice,the effects of anaesthetize on spontaneous and induced EMG were analyzed quantitatively in terms of firing rate,latency,duration and amplitude.Experimental results showed that the frequency of spontaneous EMG decreased by 50％per cycle approximately and the duration decreased,as the concentration of isoflurane increased from 0.4％to 0.75％,indicating an inhibitory state.The induced-EMG success rates of TUS/TMAS both decreased by 50％and 70％respectively,with an average increase of 0.1 s in latency and a decrease of 0.3 s and 0.5 s in duration respectively,suggesting that the modulatory effect of TUS/TMAS on the motor cortex attenuated as the depth of anesthesia increased.According to the observed correlation between the firing rate and duration of spontaneous and induced EMG,it is rational to infer that the suppression of spontaneous EMG in mice under anesthesia was one of the factors contributing to the attenuated modulatory effect.
NETL
NSTL
万方数据
