Correlation analysis of GPIba and COX-1 gene polymorphisms and aspirin resistance in high-risk population with preeclampsia
Objective To investigate the correlation between GPIba and COX-1 gene polymorphisms and aspirin resis-tance in high-risk population with preeclampsia.Methods Totally 77 patients who met the admission criteria in our hospital during 12-20 weeks of pregnancy from March 2022 to March 2023 were selected as the study subjects.They were orally administered 100mg aspirin enteric-coated tablets for 7-14 days,and two tubes of venous blood was drawn(4 ml for each)on an fasting stomach.They were divided into aspirin resistance group(AR)and sensitive group(AS)by using thromboelastography.Real-time fluorescence quantitative PCR was used to detect the expression of GPIba(GP1BA,rs6065)and COX-1(PTGS1,rs10306114)genes.PCR amplification and direct sequencing were used to an-alyze the polymorphisms of the two genes,and related clinical indicators were collected.Results There was no signifi-cant difference in baseline data or PE incidence between the two groups.Compared with AS,the expression of GP1BA and PTGS1 in AR group was significantly up-regulated(P＜0.01),but there was only one genotype of rs10306114 and three genotypes of rs6065,and there was no statistical difference in the distribution of genotypes of rs6065 between the two groups.Compared with sensitive group,AA and AA inhibition rate were significantly decreased,and AA content in platelets was significantly increased,the difference being with statistical significance.Conclusion GP1BA and PTGS1 are significantly upregulated in AR,which suggests that aspirin resetance is somewhat correlated with the two genes in PE.However,the polymorphisms at rs10306114 site of COX-1 gene and rs6065 site of GPIba gene are not correlated with the occurrence of AR.It is thought that the influence of single gene mutation is too little to be detected in rela-tively small amount of samples.
万方数据
维普
