In order to determine the time to obtain the hybrid bi-genotype strains of Morchella,six mono-genotype strains of Morchella eximia and four mono-genotype strains of Morchella sextelata were selected to hybridize at different temperatures and under different conditions.PCR technology was used to detect the genotypes of hybrid strains at the 1st,3rd,5th and 7th day.The results showed that the hybridization success rate of M.eximia is higher,and the bi-genotype strains could be obtained under different conditions after 3 days.The hybridization of M.sextelata is not stable,and the success rate of hybridization after 5 days is higher.
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