首页|羊肚菌菌株杂交条件初探

羊肚菌菌株杂交条件初探

扫码查看
为了确定获得羊肚菌杂交双基因型菌株的时间,选择了 6 个七妹羊肚菌单基因型菌株和 4 个六妹羊肚菌单基因型菌株,分别在不同温度和不同状态下杂交,利用PCR技术在第 1、3、5和 7d检测杂交菌株的基因型.结果表明,七妹羊肚菌杂交成功率更高,不同条件下 3d基本能获得双基因型菌株;六妹羊肚菌杂交不太稳定,杂交 5d成功率较高.
Preliminary Study on Hybridization Conditions of Morchella
In order to determine the time to obtain the hybrid bi-genotype strains of Morchella,six mono-genotype strains of Morchella eximia and four mono-genotype strains of Morchella sextelata were selected to hybridize at different temperatures and under different conditions.PCR technology was used to detect the genotypes of hybrid strains at the 1st,3rd,5th and 7th day.The results showed that the hybridization success rate of M.eximia is higher,and the bi-genotype strains could be obtained under different conditions after 3 days.The hybridization of M.sextelata is not stable,and the success rate of hybridization after 5 days is higher.

MorchellaBi-genotype strainshybridizationmolecular identification

唐禹婷、马宏、鲁铭颖、张淑曼、张根伟、刘振国、刘昆昂

展开 >

河北省科学院生物研究所,河北 石家庄 050081

羊肚菌 双基因型菌株 杂交 分子鉴定

河北省重点研发计划河北省科学院科技项目河北省科学院科技项目河北省科学院科技项目

22326902D213112231123311

2024

中国食用菌
中国食用菌协会 中华全国供销合作总社昆明食用菌研究所 全国食用菌科技情报中心站

中国食用菌

影响因子:0.574
ISSN:1003-8310
年,卷(期):2024.43(1)
  • 15