Cloning and Expression Analysis of 3-hydroxy-3-methylglutaryl-CoA Reductase(HMGR)Gene of Pleurotus pulmonarius
In order to obtain the full-length 3-hydroxy-3-methylglutaryl-CoA reductase(HMGR)gene and analyze the gene expression differences Pleurotus pulmonarius in normal temperature and heat stress condition,the full-length HMGR gene(named Pphg1)was isolated through RNA sequencing,and qRT-PCR was used to detect the gene expression levels in different temperature treatment of P.pulmonarius.The full-length of Pphg1 has an open reading frame of 4 059 bp.The deduced amino acid sequence of Pphg1 has 1 352 amino acid residues which form a 144.04 kDa polypeptide.qRT-PCR indicated that Pphg1 was expressed at a high level in high temperature treatment.The cDNA encoding HMGR gene from P.pulmonarius is cloned and reported for the first time.Pphg1 is supposed to be the crucial enzyme in responding to heat stress.This study provides a scientific basis for exploring the development mechanism and its molecular breeding of P.pulmonarius.
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