首页|秀珍菇黄斑病病原菌的生物学特性及药剂室内毒力测定

秀珍菇黄斑病病原菌的生物学特性及药剂室内毒力测定

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为明确秀珍菇黄斑病病原菌奈氏西地西菌的生物学特性,测定不同药剂在室内的毒力.以秀珍菇黄斑病病原菌奈氏西地西菌为试材,采用生长量法研究了温度、pH、NaCl浓度对病原菌生长的影响;采用平板抑菌圈法研究了病原菌对不同抗生素的敏感性,并测定了 14 种杀菌剂在室内的毒力.结果显示病原菌生长的最适温度为 28℃,最适pH为 7.0;在NaCl浓度为 1.0%条件下病原菌生长最好,NaCl浓度为 9.0%以上则停止生长.54℃为病原菌致死临界线.病原菌对氨苄青霉素、四环素、链霉素、硫酸核糖霉素、土霉素均敏感,其中对土霉素最敏感,对红霉素和庆大霉素不敏感.辛菌胺醋酸盐、乙蒜素、氯溴异氰尿酸、噻霉酮微乳剂、农用链霉素、中生菌素对病原菌均有抑制作用,其中中生菌素毒力最强.通过试验明确了病原菌的生物学特性,测定了药剂室内毒力,为田间病害发生规律研究和防治提供了理论依据.
Biological Properties of Pathogen Yellow Spot Disease in Pleurotus pulmonarius and Toxicity Test of Different Fungicides in Laboratory
In order to clarify the biological properties of Cedecea neteri,which causes yellow spot disease in Pleurotus pulmonar-ius,and toxicity test of different fungicides in laboratory were studied.The pathogen bacteria of yellow spot disease in P.pul-monarius was used as the test material.The effects of temperature,pH and NaCl concentration on the growth of pathogenic bac-teria were studied by growth method.The sensitivity of pathogenic bacteria to different antibiotics was studied by plate bacterio-static circle method.The bacteriostatic effects of 14 fungicides were compared in room by plate bacteriostatic circle method.The results showed that the optimum temperature of pathogen grow was 28℃,and the optimum pH was 7.0.At the NaCl concentra-tion of 1.0%,the growth of pathogen was the best,and the growth stopped when the NaCl concentration was higher than 9.0%.54℃is the critical lethal boundary of pathogenic bacteria.The pathogen was sensitive to ampicillin,tetracycline,streptomycin,ribomycin sulfate and oxytetracycline,among which oxytetracycline was the most sensitive.The pathogen was not sensitive to erythromycin and gentamicin.Occidentamine acetate,ethyl allicin,chlorobromoisocyanuric acid,thiobacillidone microemulsion,agricultural streptomycin and mesocintin had inhibitory effects on pathogenic bacteria,among which mesocintin had the highest toxicity.The biological characteristics of the pathogen were identified and antimicrobial agents were screened,which provided a theoretical basis for the study on the occurrence law and control of yellow spot disease.

Pleurotus pulmonariusyellow spot diseaseCedecea neteribiological characteristicsfungicides screening

刘增亮、吴圣进、陈雪凤、胡虹娇、张雯龙、祁亮亮、陈孝玉龙

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广西壮族自治区农业科学院微生物研究所,广西 南宁 530007

广西壮族自治区农业技术推广站,广西 南宁 530022

贵州大学农学院,贵州 贵阳 550025

秀珍菇 黄斑病 奈氏西地西菌 生物学特性 药剂筛选

广西科技厅科技基地和人才专项广西农科院科技发展基金广西农科院基本科研业务费专项广西农业科技自筹经费项目国家食用菌产业技术体系建设项目

桂科AA21196003桂农科2022JM54桂农科2024YP076Z2019109CARS-20

2024

10.13629/j.cnki.53-1054.2024.01.011

中国食用菌
中国食用菌协会　中华全国供销合作总社昆明食用菌研究所　全国食用菌科技情报中心站

中国食用菌

影响因子：0.574
ISSN：1003-8310
年,卷(期)：2024.43(1)
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