Identification of Monokaryon Mating Type in Taiwanofungus camphoratus Based on ISSR-PCR System
The monokaryon of Taiwanofungus camphoratus was obtained by protoplast monokaryon technique,and the sequence was identified based on internal transcribed interval(ITS),14 inter-simple sequence repeats(ISSR)markers were used to select clear and reproducible primers for mating type identification of monokaryon of T.camphoratus.The results showed that 31 monokaryons were obtained by protoplast monokaryon technique,and the monokaryons were identified as T.camphoratus by ITS sequence identification,14 ISSR primers were screened by using the DNA of monokaryons S2,S10,S14,S27 as templates,and 4 primers P7,P9,P21 and P25 with clear bands and good repeatability were obtained for mating type identification.Monokary-on S9 and S25 are of the same mating type and of different mating type from S2,and it was further verified by microscopic ex-amination that monokaryon S9 and S25 could form binucleate strains with lock association with S2.This method can shorten the identification time of monokaryon mating type of T.camphoratus.
万方数据
维普
