Trichoderma resistance is an important index for screening in Lentinus edodes breeding.In order to improve the screening efficiency of strain resistance to Trichoderma,three screening methods were used to against Trichoderma,which were width of antagonistic band,antagonistic radius of L.edodes,and invasion distance of Trichoderma.Using the method of con-frontation culture between L.edodes strains and Trichoderma strains in petri dishes,the resistance of 256 L.edodes hybrid strains to Trichoderma were screened.61 strains with strong resistance(a total of 101 strains)were screened out by each method.Then the strains were cultivated.The consistency,correlation and reliability of these three methods were compared.The results showed that the trend lines of the three methods were consistent,and the correlation coefficients were-0.699,0.536 and-0.675,respectively.The correlation coefficient showed that there was a very significant and large correlation between these three groups of data.The population indexes of cultivate pollution were 6.3,4.6 and 9.9,respectively.The results showed that the strain screened by the method of antagonistic band width had better resistance to Trichoderma,followed by the method of antag-onistic radius of L.edodes,and the worst method was Trichoderma invasion distance.
关键词
香菇菌株/抗木霉/拮抗带宽度/香菇拮抗半径/木霉入侵距离
Key words
Lentinus edodes strain/Trichoderma resistant/antagonistic band width/antagonistic radius of Lentinus edodes/Tri-choderma invasion distance
维普
万方数据