A rapid detection method for the determination of Ganoderma polysaccharides by using 1H nuclear magnetic reso-nance spectroscopy(1H-NMR)was established and optimized.The heterotopic hydrogen region(8 4.42-5.40 ppm)was used as the'fingerprint'identification area to determine the Ganoderma polysaccharide by comparing the similarity between the sample spectrum and the standard spectrum,and the methodology verification was carried out.8 batches of self extracted Ganoderma lucidum polysaccharides were identified using this method,and the results showed a similarity of over 0.95 with the standard sample spectrum,demonstrating good reproducibility.The established 1H-NMR rapid detection method can quickly and accu-rately detect Ganoderma polysaccharides,and has the characteristics of simple operation,good repeatability,strong specificity and high accuracy.
关键词
灵芝多糖/核磁共振氢谱法/结构鉴定
Key words
Ganoderma polysaccharides/nuclear magnetic resonance hydrogen spectroscopy/structural identification
维普
万方数据