The development of Tricholoma matsutake is closely related to the ecological and environmental characteristics of the soil.In this experiment,we collected the shiro soil from'T.matsutake Nanhua'shiro(the China geographical indication prod-uct),and compared the differences in physicochemical and the number of culturable microorganisms between the shiro soil and non-shiro soil,and contrasted the microbial diversity and microbial community structure which performed high-throughput se-quencing,to clarify the characteristics of the soil microflora.The results showed that the physicochemical of the shiro soil are higher than the non-shiro soil,such as potassium(raised 151.22%),manganese(raised 156.38%),cuprum(raised 105.88%)content showed extremely significant differences;and phosphorus,zinc,magnesium content showed significant differences.There were extremely significant differences in the number of three major microbial groups in the shiro soil compared with the control.The high-throughput sequencing results revealed significantly different microbial community structures of the shiro soil.The dominant bacteria were Burkholderia-Caballeronia-Paraburkholderia,Mycobacterium,Acidipila,Acidothermus.The dominant fungi were Chloridium,Sagenomella,Glarea,unclassified-Clavulinaceae and Tricholoma.