摘要
野生松茸的发生与其土壤的生态环境特点密切相关.试验以国家地理标志产品"南华松茸"为对象,比较菌塘土壤与非菌塘土壤的理化性质及可培养微生物数量,并基于高通量测序技术分析土壤微生物多样性及菌群结构差异,以明确菌塘的微生态区系特征.结果表明,菌塘土壤各理化参数高于非菌塘土,其中速效钾(含量增加151.22％)、锰(增加156.38％)、铜(增加105.88％)含量差异极显著,磷、锌、镁含量差异显著.菌塘土壤中主要三大微生物类群的数量与非菌塘相比差异极显著;高通量测序结果表明菌塘土壤微生物群落结构差异显著,优势细菌类群为卡氏伯克霍尔德菌属(Burkholderia-Caballeronia-Paraburkholderia)、分支杆菌属(Mycobacterium.)、酸杆菌属(Acidipila)、热酸菌属(Acidothermus);优势真菌类群为暗梗单孢霉属(Chloridium)、Sagenomella、Glarea、锁瑚菌科未分类的属 unclassified-Clavulinaceae、口蘑属(Tricholoma).
Abstract
The development of Tricholoma matsutake is closely related to the ecological and environmental characteristics of the soil.In this experiment,we collected the shiro soil from'T.matsutake Nanhua'shiro(the China geographical indication prod-uct),and compared the differences in physicochemical and the number of culturable microorganisms between the shiro soil and non-shiro soil,and contrasted the microbial diversity and microbial community structure which performed high-throughput se-quencing,to clarify the characteristics of the soil microflora.The results showed that the physicochemical of the shiro soil are higher than the non-shiro soil,such as potassium(raised 151.22％),manganese(raised 156.38％),cuprum(raised 105.88％)content showed extremely significant differences;and phosphorus,zinc,magnesium content showed significant differences.There were extremely significant differences in the number of three major microbial groups in the shiro soil compared with the control.The high-throughput sequencing results revealed significantly different microbial community structures of the shiro soil.The dominant bacteria were Burkholderia-Caballeronia-Paraburkholderia,Mycobacterium,Acidipila,Acidothermus.The dominant fungi were Chloridium,Sagenomella,Glarea,unclassified-Clavulinaceae and Tricholoma.
基金项目
云南省科技重大专项(202102AE090051)
云南省刘庆洪专家工作站项目(202305AF150164)
万方数据