To clarify the bioactive components contents and functions of the fermentation product from Auricularia heimuer,the contents of protein,fiber,polysaccharide,melanin and flavonoid,as well as the antioxidant and anticancer activities of the freeze-dried powder of A.heimuer Y9 liquid fermentation product were determined.The results showed that,in A.heimuer Y9 fermentation product freeze-dried powder,the crude protein content was 135.04 mg·g-1,the crude fiber content was 39.24 mg·g-1,the crude polysaccharide content was 77.04 mg·g-1,the melanin content was 2.80 mg·g-1,and the total flavonoid content was 4.72 mg·g-1.The IC50 values of DPPH·,O2·-and OH scavenging rates of the fermentation product freeze-dried powder were 4.002、4.345 and 8.623 mg·mL-1,respectively.The IC50 values of the inhibition rates against gastric cancer cells(BGC-823)and breast cancer cells(MCF-7)were 9.775 and 3.995 mg·mL-1,respectively.The fermentation product were rich in bioactive com-ponents with strong antioxidant and antitumor activities.