粗毛纤孔菌和瓦尼桑黄液体共同发酵工艺优化及胞外多糖抗肿瘤活性分析
Optimization of Co-fermentation Process of Sanghuangporus vaninii and Inonotus hispidus and the Anti-tumor Activity Analysis of Extracellular Polysaccharide
杨祎 1雷潇 1解丽娟 1胡广文 1周冰玉 1周宇 1唐少军1
作者信息
- 1. 湖南省微生物研究院,湖南 长沙 410009
- 折叠
摘要
为提高粗毛纤孔菌和瓦尼桑黄液体共同发酵条件下胞外多糖的产量和活性,利用单因素试验结合正交试验优化粗毛纤孔菌和瓦尼桑黄液体共同发酵的工艺,并通过MTT试验分析胞外多糖的抗肿瘤活性.获得粗毛纤孔菌和瓦尼桑黄的液体共同发酵最佳工艺:培养基配方为葡萄糖40 g·L-1、酵母粉10 g·L-1、蛋白胨10 g·L-1、MgSO4·7H2O1g·L-1、KH2PO4 1.5 g·L-1、VB1 0.05 g·L-1,pH 7.0,转速 180 r·min-1,培养温度为 26 ℃,培养时间为12d.在该工艺条件下进行共同发酵,胞外多糖和菌丝产量分别达到2.86 g·L-1和32.9g·L-1,均高于2个菌株单独发酵的产量,且共同发酵产生的多糖对海拉细胞抑制活性明显高于单菌种发酵.本研究中探索了 1种新的瓦尼桑黄和粗毛纤孔菌发酵模式,提升了瓦尼桑黄和粗毛纤孔菌胞外总多糖的产量和抗肿瘤活性,为大型真菌多糖产品的开发提供了物质基础.
Abstract
In order to improve the production and activity of extracellular polysaccharide(EXP)by optimizing the co-fermenta-tion process of Sanghuangporus vaninii and Inonotus hispidus,the mycelia and extracellular polysaccharide yield were used as index,the co-fermentation process was optimized based on single factor test and orthogonal test,and the inhibitory activi-ty of exopolysaccharide on tumor cells was analyzed by MTT test.The results showed that,the best process of co-fermenta-tion of S.vaninii and I.hispidus is glucose 40 g·L-1,peptone 10 g·L-1,yeast 10 g·L-1,MgSO4·7H2O 1 g·L-1,KH2PO4 1.5 g·L-1,VB1 0.05 g·L-1,pH 7.0,culture speed 180 r·min-1,culture temperature 26 ℃,culture time 12 d.Under this process condition,the yields extracellalar polysaccharide and mycelia reached 2.86 g·L-1and 32.9 g·L-1 respectively,both were higher than the yields of two single strains.The inhibitory activity of polysaccharide produced by co-fermentation on HeLa cells was significant-ly higher than that of single strain fermentation.In this study,a new fermentation mode of S.vaninii and I.hispidus was ex-plored to improve the extracellular polysaccharide yield and anti-tumor activity,which provided material basis for the develop-ment of macrofungal polysaccharide products.
关键词
粗毛纤孔菌/瓦尼桑黄/共同发酵/工艺优化/抗肿瘤Key words
Sanghuangporus vaninii/Inonotus hispidus/co-fermentation/process optimization/anti-tumor引用本文复制引用
基金项目
湖南省自然科学基金(2022JJ40233)
长沙市重点研发计划项目(kh2201216)
出版年
2024
NETL
NSTL
万方数据