Optimization of Co-fermentation Process of Sanghuangporus vaninii and Inonotus hispidus and the Anti-tumor Activity Analysis of Extracellular Polysaccharide
In order to improve the production and activity of extracellular polysaccharide(EXP)by optimizing the co-fermenta-tion process of Sanghuangporus vaninii and Inonotus hispidus,the mycelia and extracellular polysaccharide yield were used as index,the co-fermentation process was optimized based on single factor test and orthogonal test,and the inhibitory activi-ty of exopolysaccharide on tumor cells was analyzed by MTT test.The results showed that,the best process of co-fermenta-tion of S.vaninii and I.hispidus is glucose 40 g·L-1,peptone 10 g·L-1,yeast 10 g·L-1,MgSO4·7H2O 1 g·L-1,KH2PO4 1.5 g·L-1,VB1 0.05 g·L-1,pH 7.0,culture speed 180 r·min-1,culture temperature 26 ℃,culture time 12 d.Under this process condition,the yields extracellalar polysaccharide and mycelia reached 2.86 g·L-1and 32.9 g·L-1 respectively,both were higher than the yields of two single strains.The inhibitory activity of polysaccharide produced by co-fermentation on HeLa cells was significant-ly higher than that of single strain fermentation.In this study,a new fermentation mode of S.vaninii and I.hispidus was ex-plored to improve the extracellular polysaccharide yield and anti-tumor activity,which provided material basis for the develop-ment of macrofungal polysaccharide products.
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