羊肚菌连作障碍土壤中病原微生物多样性分析
Soil Pathogenic Microbial Diversity Analysis of Morchella spp.Continuous Cropping Obstacle
李雪松 1孙达锋 2华蓉 3李建英 2岳万松 2刘绍雄2
作者信息
- 1. 中华全国供销合作总社昆明食用菌研究所,云南昆明 650221;云南省食用菌产业发展研究院,云南昆明 650221
- 2. 中华全国供销合作总社昆明食用菌研究所,云南昆明 650221
- 3. 云南省食用菌产业发展研究院,云南昆明 650221
- 折叠
摘要
以羊肚菌连作障碍土壤为试验材料,采用高通量扩增子测序技术和生物信息学方法分析病害土壤中细菌和真菌的多样性,以期为羊肚菌栽培技术优化和连作病害防治提供参考依据.结果表明,病害羊肚菌土壤优势细菌类群有弧菌属(Vibrio)、疣微菌科UCG-005(Ruminococcaceaea UCG-005)等;优势真菌类群中的拟青霉属(Paecilomyces)、镰刀菌属(Fusarium)、木霉属(Trichoderma)等均为大型真菌病原菌的相关类群.病原细菌和病原真菌的共同作用是造成羊肚菌患白霉病、枯萎病、柄腐病等连作病害的原因.
Abstract
The continuous cropping obstacle soil in Morchella spp.cultivation was used as experiment material.Using high throughput amplicon sequencing technology and bioinformation to analyze the diseased soil bacterial and fungal diversity,in or-der to provide a reference basis for optimization of culture Morchella spp.and disease control.The results showed that Vibrio and Ruminococcaceaea UCG-005 and so on from the bacterial dominant group of diseased soil are related groups of macrofungi pathogens.Among the dominant fungal groups,Paecilomyces,Fusarium and Trichoderma are also related groups of macrofungi pathogens.The common action of pathogenic bacteria and pathogenic fungi is the cause of Morchella spp.suffering from white mildew,fusarium wilt,stalk rot and other continuous cropping diseases.
关键词
羊肚菌/连作障碍/细菌多样性/真菌多样性Key words
Morchella spp./continuous cropping obstacle/bacterial diversity/fungal diversity引用本文复制引用
基金项目
云南省创新引导与科技型企业培育计划(202304BP090011)
云南省科技人才与平台计划(202305AD160051)
云南省创新引导与科技型企业培育计划(202304BI090029)
出版年
2024
NETL
NSTL
万方数据