Disease Type Investigation and Pathogens Identification of Pleurotus pulmonarius
In recent years,the frequent occurrence of diseases has caused a serious impact on the yield and quality of Pleurotus pulmonarius,so it is very important to identify and study the characteristics of the pathogens.Pathogens were isolated and puri-fied from diseased samples collected in different periods.Koch's rule,microscopic characteristics,ITS or 16S rRNA sequencing were used to identify the pathogens.The results showed that the main fungal pathogens were Trichoderma,Penicillium,Strep-tospora,Mucor,Chaetomium,which caused green mold disease,red powder disease and so on.Fungal diseases mostly occurred in the vegetative stage of P.pulmonarius.Bacterial pathogens mainly include Pseudomonas,Ewingella,Burkholderia,Stenotrophomonas and Chitinophaga,which caused yellow mushroom disease and wilt disease.Bacterial diseases mostly occur in the reproductive growth period of P.pulmonarius.
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