1株野生血红栓菌的分离鉴定及生物学特性分析
Isolation,Identification and Biological Characteristics Analysis of a Wild Strain of Trametes sanguinea
王南南 1王付彬 1刘长安 1王玉国 1张琴 1高秋荣 2张连秋1
作者信息
- 1. 济宁市农业科学研究院,山东济宁 272031
- 2. 济宁市嘉祥县黄垓镇农业技术推广站,山东济宁 272405
- 折叠
摘要
从曲阜孔林采集到1株野生担子菌子实体,通过组织分离获得其菌丝,编号为YEF202102.进行了形态学特征分析、ITS测序及系统发育树构建,发现菌株YEF202102的形态特征与血红栓菌相符,与血红栓菌ITS序列相似度达到99％,并与其在系统发育树上聚在一支,自聚值为100％,鉴定为血红栓菌(Trametes Sanguinea).为开发利用该野生资源,通过单因素试验和正交试验探究了菌株YEF202102在固体培养条件下各因素对菌丝生长的影响.结果显示,最适合菌株YEF202102菌丝生长的条件为麦芽糖20 g·L-1、酵母粉2 g·L-1,pH 5.0,温度 25℃.
Abstract
Pure culture strain(numbered as YEF202102)was from the wild fruit bodies of basidiomycete from the Confucian Cemetery,Qufu City.Through morphological characteristics analysis,ITS sequencing and phylogenetic tree construction,the re-sults showed that the morphological characteristics of this strain were consistent with that of Trametes.sanguinea,and the simi-larity of ITS sequence with T.sanguinea reached 99％,and the strain was clustered in the phylogenetic tree with a self-aggrega-tion value of 100％.It was therefore identified as T.Sanguinea.The biological characteristics of this fungus were studied.The results showed that,the best condition for mycelia growth of strain YEF202102 was fructose 20 g·L-1,yeast paste 2 g·L-1,pH 5.0 and temperature 25℃.
关键词
血红栓菌/分离/鉴定/生物学特性Key words
Trametes sanguinea/isolation/identification/biological characteristics引用本文复制引用
基金项目
山东省现代农业产业技术体系食用菌创新团队建设项目(SDAIT-07-12)
出版年
2024
NETL
NSTL
万方数据