Identification and Cultivation of a Wild Pleurotus ostreatus
Morphological and molecular biology identification were conducted on a wild strain(PA062844)collected from fallen Populus in Kunyu Mountain,Yantai,Shandong Province,and further artificial domestication and cultivation were carried out.The results showed that the fruit bodies cap of this wild strain was shell-shaped,gray-white in color;partial in stipe,white;and white in gills,which was preliminarily identified as Pleurotus ostreatus.The ITS sequence of the wild strain was amplified and subjected to BLAST analysis in GenBank.The results showed that the sequence had high homology with multiple ITS sequences of P.ostreatus.Combined with morphological identification results,the wild strain was identified as P.ostreatus.There were sig-nificant differences in mycelial growth rate and dry weight between wild strain and control P.ostreatus(LD-1)on PDA medium,and the yield of wild strain was significantly lower than that of control P.ostreatus.Thus further domestication and cultivation are needed.
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