1株野生平菇的鉴定及栽培
Identification and Cultivation of a Wild Pleurotus ostreatus
金永峰 1杨家乐 1朱道雷 1王磊 2隋良缘 1潘惠中 1盖宇鹏 1程显好 3李维焕3
作者信息
- 1. 鲁东大学农学院/烟台市食药用菌技术创新中心,山东烟台 264025
- 2. 山东兴程生态农业科技有限公司,山东烟台 264025
- 3. 鲁东大学农学院/烟台市食药用菌技术创新中心,山东烟台 264025;国家盐碱地综合利用技术创新中心,山东东营 257345
- 折叠
摘要
对采自山东省烟台昆嵛山杨树倒木上的1株野生菌株(PA062844)进行形态学和分子生物学鉴定,并进一步开展人工驯化与栽培.结果表明,该野生菌株子实体菌盖呈贝壳状、颜色为灰白色,菌柄偏生、白色,菌褶白色延生,初步鉴定为侧耳属平菇.扩增该野生菌株的ITS序列,在GenBank中进行BLAST分析,结果显示该序列与多条平菇ITS序列同源性较高,结合形态特征将该野生菌株鉴定为平菇.野生菌株与对照菌株(LD-1)在PDA培养基上的菌丝生长速度、干质量有显著差异,且野生菌株产量显著低于对照菌株,还需进一步驯化栽培.
Abstract
Morphological and molecular biology identification were conducted on a wild strain(PA062844)collected from fallen Populus in Kunyu Mountain,Yantai,Shandong Province,and further artificial domestication and cultivation were carried out.The results showed that the fruit bodies cap of this wild strain was shell-shaped,gray-white in color;partial in stipe,white;and white in gills,which was preliminarily identified as Pleurotus ostreatus.The ITS sequence of the wild strain was amplified and subjected to BLAST analysis in GenBank.The results showed that the sequence had high homology with multiple ITS sequences of P.ostreatus.Combined with morphological identification results,the wild strain was identified as P.ostreatus.There were sig-nificant differences in mycelial growth rate and dry weight between wild strain and control P.ostreatus(LD-1)on PDA medium,and the yield of wild strain was significantly lower than that of control P.ostreatus.Thus further domestication and cultivation are needed.
关键词
平菇/ITS序列分析/生物学特性/栽培Key words
Pleurotus ostreatus/ITS sequential analysis/biological characteristics/cultivation引用本文复制引用
基金项目
山东省科技型中小企业创新能力提升工程项目(2023TSGC900)
山东省科技型中小企业创新能力提升工程项目(2021TSGC1233)
济宁市重点研发计划(2023NYNS005)
山东省现代农业产业技术体系食用菌创新团队遗传育种岗位(SDAIT-07-03)
出版年
2024
NETL
NSTL
万方数据