Preparation and Comparison of Protoplast Regeneration Strains of Morchella sextelata
The protoplast formation and regeneration conditions of Morchella sextelata were studied,the mycelium growth rates of the regenerated strains was measured and the cultivation experiment was carried out.The results showed that the optimum condition for protoplast formation was to select the mycelium cultured for 3 d,use 0.6 mol·L-1 mannitol as osmotic stabilizer,add 1.5％lyticase B,and the enzymolysis was conducted at 30 ℃ for 3 h.Under this condition,the protoplast yield reached 8x 104 per mL.The regenerated strain had great differents from the original strain in mycelia morphology,cell structure and mycelial growth rate.The mycelium growth rate of regenerated strain Z2,Z4 groups in different media was faster,and during cultivation the yield reached 427.3 g·m-2 and 380.3 g·m-2 respectively,which could be used as germplasm resources for further investigation.
NETL
NSTL
万方数据
