Determination of Total Sugar Content in Pleurotus citrinopileatus by Graphite Digestion and Spectrophotometry
The Pleurotus citrinopileatus of total sugar yield were determinated by graphite digestion,and the hydrolysis condi-tions of graphite digestion were optimized by hydrochloric acid concentration,hydrochloric acid addition,hydrolysis temperature and hydrolysis time.The results showed that the highest total sugar yield was obtained when the hydrochloric acid concentration was 3 mol·L-1,the volume of hydrochloric acid was 40 mL,the hydrolysis temperature was 140 ℃,and the hydrolysis time was 120 min.Methodological validation showed good precision and accuracy.The measured value was within the reference range for the certified reference materials,which verified the feasibility of this method.
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