石墨消解仪-分光光度法测定金顶侧耳中的总糖含量
Determination of Total Sugar Content in Pleurotus citrinopileatus by Graphite Digestion and Spectrophotometry
曹燕妮 1华蓉 2刘韵然 1游金坤 3王娟 3杨璐敏 3孙达锋2
作者信息
- 1. 云南省食用菌产业发展研究院,云南昆明 650221
- 2. 云南省食用菌产业发展研究院,云南昆明 650221;中华全国供销合作总社昆明食用菌研究所,云南昆明 650221
- 3. 中华全国供销合作总社昆明食用菌研究所,云南昆明 650221
- 折叠
摘要
试验以盐酸浓度、盐酸添加量、水解温度、水解时间优化石墨消解仪水解条件,测定了金顶侧耳中的总糖得率.结果表明,盐酸浓度为3 mol·L-1、盐酸体积为40 mL、水解温度为140℃、水解时间为120 min时,金顶侧耳中总糖得率最高,方法学验证其具有良好的精密度和准确度.对有证标准物质的实测值在参考范围内,验证了此方法的可行性.
Abstract
The Pleurotus citrinopileatus of total sugar yield were determinated by graphite digestion,and the hydrolysis condi-tions of graphite digestion were optimized by hydrochloric acid concentration,hydrochloric acid addition,hydrolysis temperature and hydrolysis time.The results showed that the highest total sugar yield was obtained when the hydrochloric acid concentration was 3 mol·L-1,the volume of hydrochloric acid was 40 mL,the hydrolysis temperature was 140 ℃,and the hydrolysis time was 120 min.Methodological validation showed good precision and accuracy.The measured value was within the reference range for the certified reference materials,which verified the feasibility of this method.
关键词
金顶侧耳/总糖/石墨消解/水解Key words
Pleurotus citrinopileatus/total sugar/graphite digestion/hydrolysis引用本文复制引用
基金项目
科技人才与平台计划(院士专家工作站)(202305AF150187)
科技人才与平台计划(202405AD350043)
出版年
2024
NETL
NSTL
万方数据