According to the climate characteristics of southern China and the growth characteristics of Stropharia rugosoannulata,the key techniques were summarized from the aspests of the site selection,fruiting facility construction,culture production,cul-ture medium formula,aerobic fermentation of substrate,subatrate spreading on shelf,seeding,spawn-running management and fruiting management and so on,in order to provide support for the development of the layered cultivation of Stropharia rugosoan-nulata in southern China.