Genetic Diversity Analysis and Strains Screening of Auricularia auricula with High Polysaccharide Yield
The genetic diversity of 45 Auricularia auricula germplasm resources collected from three northeastern provinces was analyzed by somatic incompatibility analysis and ISSR molecular marker,then high yield polysaccharide strains of which were screened by the liquid deep fermentation technology.The results showed that 9 strains had no obvious antagonistic reaction and were closely related to each other.The genetic similarity coefficient of the other 36 strains ranged from 0.44 to 0.96,and the genetic background was rich.When the genetic similarity coefficient was 0.6,the tested strains were clustered into nine groups:Heishan,Heifeng,Heifeng 2,Shen 8-7,Heiwei Banjin,Heiwei-15,Heibao No.1,2016-A,2016-B,2016-C,2016-E,2019-013,2019-192,Xinke No.10,Heihouyuan,Huateng No.2,Huateng No.4 and Chunbao were the first group;Mu 29,Changbai 7,Xinke No.1 and 919 were the second group;Gaoyouwang,1022,2019-003 and 918 were the third group;Xunfenghei,Yanfeng-13,Banjin S1908,888 and Fengchanwang were the fourth group;The genetic similarity coefficient of Jingfa 13,89-1,Fengshou No.2,2019-199 and Haiyuan was less than 0.6,indicating that the genetic relationship was distant,and the single strain as sui generis,which were the fifth to the ninth groups respectively.From these strains,A.auricula strain Heifeng 2 with high polysaccharide content and high yield was screened out.
Auricularia auriculaISSR molecular markergenetic diversitypolysaccharidethree northeastern provinces
万方数据
维普
