Comparative Transcriptomic Analysis of Mycelia and Primordium of Thelephora ganbajun Zang
Thelephora sp.,an esteemed wild edible fungus in Yunnan Province,has yet to fully disclose its molecular develop-mental mechanisms.Leveraging the G46 genome of Thelephora ganbajun Zang as a reference,transcriptome sequencing and analysis were conducted on its mycelium and primordium.The analysis uncovered 4 197 differentially expressed genes(DEG),with 2 051 upregulated and 2 146 downregulated.GO enrichment analysis highlighted significant enrichment of these DEG in biological processes related to metabolism,cellular processes,and single-organism activities;cellular components including cell,cell part,and organelle part;and molecular functions such as catalytic activity,binding,and transporter activity.KEGG pathway enrichment analysis further revealed that the MAPK signaling pathway,protein processing in the endoplasmic reticulum,and amino sugar and nucleotide sugar metabolism pathways were enriched among the DEG.Notably,DEG involved in protein pro-cessing in the endoplasmic reticulum and amino sugar and nucleotide sugar metabolism were predominantly up regulated and expressed during the prophase.Additionally,the expression of 1 742 DEG associated with carbohydrate-active enzymes(CAZymes)was observed,with the carbohydrate-binding modules(CBMs)and glycoside hydrolases(GHs)families being the most abundant,accounting for approximately 40.01％ and 29.56％ respectively.These genes played a pivotal role in sugar sub-strate degradation,indicating a substantial demand for materials and energy during primordium formation.The enrichment of genes associated with lignocellulose degradation also suggests a potential close relationship between the development of T.gan-bajun primordia and its symbiotic plants.
Thelephora ganbajunprimordial formationtranscriptomegrowth and developmentectomycorrhizal fungi
万方数据
维普
