首页|不同生长阶段紫灵芝中多糖、三萜和甾醇的含量测定及其抗氧化活性研究

不同生长阶段紫灵芝中多糖、三萜和甾醇的含量测定及其抗氧化活性研究

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为研究紫灵芝不同生长阶段主要活性成分的含量变化及其抗氧化活性,采用UV-Vis法对紫灵芝不同生长阶段主要活性成分(多糖、三萜和甾醇)的含量进行测定,并利用DPPH和ABTS法评价其体外抗氧化活性.结果显示,从原基到开伞阶段多糖的含量呈上升趋势(0.68％～0.83％),并在开伞阶段达到最高(0.83％),然后到成熟喷粉阶段逐渐降低(0.83％～0.67％).三萜和甾醇的含量与多糖含量变化略有不同,从原基到伞下转褐阶段逐步升高(0.75％～1.14％),并在伞下转褐阶段达到最高(1.14％),然后呈现下降趋势(1.14％～1.10％).DPPH和ABTS自由基清除试验显示,紫灵芝多糖、三萜和甾醇类成分均具有较强的抗氧化活性,并与其含量正相关.试验结果表明,紫灵芝不同生长阶段多糖、三萜和甾醇的含量呈现出动态变化,并显示出良好的抗氧化作用.
Studies on the Contents and Antioxidant Activities of Polysaccharides,Triterpenoids and Sterols from Ganoderma sinense at Different Growth Stages
To study the content changes of the main active ingredients in different growth stages of Ganoderma.sinense and their antioxidant activities.Using UV-Vis spectrophotometry,the concentrations of polysaccharides,triterpenoids,and sterols in various development stages of G.sinense were ascertained.DPPH and ABTS were then utilized to investigate the antioxidant properties of these substances.The contents of polysaccharides increased from the primordium to the umbrella stage(0.68％-0.83％ )and reached the highest(0.83％ )at the umbrella stage,while the contents decreased gradually from the umbrella stage to the mature stage(0.83％-0.67％ ).The contents of triterpenoids and sterols were different from those of polysaccharides.The contents of triterpenoids and sterols gradually increased from the primary to the subumbrella browning stage(0.75％-1.14％ ),and reached the highest(1.14％ )at the subumbrella browning stage,and then began to decrease(1.14％-1.10％ ).According to the results of DPPH and ABTS scavenging studies,the polysaccharides,triterpenoids and sterols of G.sinense exhibited potent antioxidant activity and were favorably linked with their concentration.The findings demonstrated that the contents of polysac-charides,triterpenoids and sterols varied dynamically and shown strong antioxidant properties.

Ganoderma sinensedifferent growth stagespolysaccharidestriterpenoids and sterolscontent determinationan-tioxidant activity

周玉芳、刘玉琴、黄绍权、薛珺、赖闻玲、钱益平、何其房

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赣南师范大学化学化工学院,江西 赣州 341000

赣南师范大学生命科学学院,江西 赣州 341000

赣州正阳生态科技有限公司,江西 赣州 341000

紫灵芝 不同生长阶段 多糖 三萜和甾醇 含量测定 抗氧化活性

国家自然科学基金青年项目赣州市重点研发计划重点项目赣南师范大学大学生创新创业训练计划项目赣南师范大学大学生创新创业训练计划项目

22304032202101075041CX220056CX230068

2024

10.13629/j.cnki.53-1054.2024.05.012

中国食用菌
中国食用菌协会　中华全国供销合作总社昆明食用菌研究所　全国食用菌科技情报中心站

中国食用菌

影响因子：0.574
ISSN：1003-8310
年,卷(期)：2024.43(5)