In this experiment,the nutrient formula of Mangifera indica juice medium for Pleurotus ostreatus was optimized by taking the proportion of M.indica juice,the proportion of peptone and the amount of mycelium inoculation as the variables.The mycelia were cultured for 5 days,and the single variable experiment was carried out by measuring the dry weight of mycelia and the exopolysaccharide content by sulfuric acid-phenol method.At the same time,L9(33)orthogonal test was used to deter-mine the optimal formula of liquid medium.The results showed that the dry weight of mycelia increased first and then decreased with the increases of M.indica juice proportion,and the dry weight of mycelia was the highest when the proportion of M.indica juice was 40％ .The mycelial dry weight and extracellular polysaccharide content increased first and then decreased with pep-tone supplementation,and the highest value was reached when peptone supplementation was 0.6％ .The mycelial dry weight and exopolysaccharide content increased first and then decreased with the increase of inoculation amount,and the highest value was reached when the inoculation amount was 0.8％ .The orthogonal experiment showed that the optimal nutri-ent formula of P.ostreatus and M.indica juice medium was 40％ M.indica juice,0.6％ peptone and 0.8％ inoculum.
关键词
平菇菌丝体/芒果汁/液体培养/培养条件优化
Key words
Pleurotus ostreatus mycelium/Juice of Mangifera indica/liquid culture/optimization of culture conditions
维普
万方数据