Biological Characteristics and Domestication Cultivation of Wild Auricularia thailandica
Auricularia-like species growing on decaying wood in Pingbian Miao Autonomous County,Honghe Hani and Yi Au-tonomous Prefecture,Yunnan Province,were identified as Auricularia thailandica based on morphological characters and molec-ular phylogenetic analysis.Pure strain was obtained by tissue isolation,and solid-state culture,liquid-state culture,domestica-tion cultivation of this specie collected in China was carried out for the first time.The experimental results showed that under solid-state conditions,the optimal formula was 20.0 g·L-1 soluble starch,2.0 g·L-1 soybean meal,3.0 g·L-1 calcium chloride,18.0 g·L-1 agar powder,the pH was 7 and the culture temperature was 32℃.Under liquid-state conditions,the optimal formula was 20.0 g·L-1 soluble starch,2.0 g·L-1 peptone,3.0 g·L-1 dipotassium phosphate,the pH was 6 and the culture temperature was 26℃.The results of cultivation experiment showed that it's primordium differentiated quickly,the ear segments were con-sistent,the fruit bodies was thick,the ear shape was regular,and the sporulation surface was the same as the wild fruit bodies,with obvious folds,which had great application value and could be used as new germplasm resources.
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