贵州省2株野生二孢拟奥德蘑的生物学特性分析及驯化栽培
Biological Characteristics Analysis and Domestication Cultivation of Two Wild Hymenopellis raphanipes in Guizhou Province
张秀伟 1何大智 1蔡甫格 1牛力立1
作者信息
- 1. 安顺市农业科学院,贵州 安顺 561000
- 折叠
摘要
在贵州省安顺市普定县茶叶基地内采集到 2 株野生菌子实体,采用组织分离法获得纯培养菌株,结合形态学和分子生物学方法鉴定,同时开展了菌丝体生物学特性分析及驯化栽培试验.结果表明 2 个野生菌株MDCG-1 和 MDCG-2 均为二孢拟奥德蘑(Hymenopellis raphanipes).二者菌丝生长的最适温度均为 25℃,MDCG-1 菌丝培养的最适碳源为葡萄糖,最适氮源为酵母粉;MDCG-2 菌丝培养的最适碳源为蔗糖,最适氮源为蛋白胨.栽培试验表明 2个菌株均可出菇,MDCG-1、MDCG-2栽培的生物学效率分别为 33.72%、45.05%.
Abstract
Two wild fruit bodies were collected from the Puding County tea base in Anshun,Guizhou Province.The pure culture strains were obtained by tissue isolation and identified by morphological and molecular methods.At the same time,the biologi-cal characteristics of mycelium were analyzed and domesticated cultivation experiments were carried out.The results showed that the two wild strains MDCG-1 and MDCG-2 were Hymenopellis raphanipes.The optimum temperature for mycelial growth was 25℃,the optimum carbon source for culture of MDCG-1 was glucose and the optimum nitrogen source was yeast powder,the optimum carbon source for culture of MDCG-2 was sucrose and the optimum nitrogen source was peptone.Cultivation ex-periment showed that the two strains could produce fruit bodies,and the biological efficiencies of MDCG-1 and MDCG-2 were 33.72%and 45.05%respectively.
关键词
二孢拟奥德蘑/贵州省/生物学特性/驯化栽培Key words
Hymenopellis raphanipes/Guizhou Province/biological characteristics/domestication cultivation引用本文复制引用
出版年
2024
NETL
NSTL
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