Isolation,Identification and Domestication of Wild Auricularia spp.Strains in Qinling Mountains
8 wild Auricularia auricula strains in Qinling Mountains are collected,isolated and domesticated from 2022 to 2023.Results shows that 6 strains(Danfeng wild A.auricula,Shanyang wild A.auricula,Beier,Zhuer,Zhener,and Shanger)are i-dentified as A.auricula,Luoer is identified as A.villosula.Shangzhou district wild Auricularia spp.is identified as A.Fusco-succinea.DNAMAN comparison shows the similarity of 18S rDNA-ITS gene sequence of these strains is 69.58%,which indicates that the 8 strains have certain genetic relationship,but the genetic relationship is relatively distant.No registered sequences close to Zhuer and Zhener are found in the NCBI,implying that they may be new strains.From mycelium growth,fruiting emergence and yield of single cultivation bag,Shanger and Danfeng wild A.auricula are precocious,high in yield,medium in size,strong in stress resistance,good in adaptability and excellent in comprehensive performance,and can be further domesticated and cultivated.Shanyang wild A.auricula and Beier are eliminated for their weak stress resistance and poor adaptability.Zhuer is small and thin but can reach high yield,Zhener fruit bodies are darker but with poor adaptability in the later fruiting stage and can't resistant to high temperature.Thus,Zhuer and Zhener can be used as parents for hybrid breeding of A.auricula.
wild Auricularia auriculaQinling mountainsisolation and identificationdomestication test
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