Establishment of HPLC Detection Method for L-tryptophan in Edible Fungi
Methodological validation was conducted on the established high performance liquid chromatography(HPLC)method for determining L-tryptophan content in edible fungi.Using an AcclaimTM 120 C18 chromatography column(4.6 mm×250 mm,5 μm),isometric elution was performed with 0.05 mol·L-1 sodium acetate buffer(pH adjusted to 4.0-5.0 with glacial acetic acid)and pure methanol as mobile phases(the volume ratio is 70∶30),with a flow rate of 1.0 mL·min-1,detection wavelength of 280 nm,column temperature of 30℃,and injection volume of 20 μL.The results showed that L-tryptophan showed a good lin-ear relationship in the range of 4-100 μg·mL-1,and the R2>0.995 5.The relative standard deviation of precision,repeatability and stability within 24 hours is less than 15%(n=6),and the spiked recovery rate was 87.5%(relative standard deviation was 0.63%).The established HPLC detection method is simple,fast,highly sensitive,specific,and has good separation,it is suitable for the determination of L-tryptophan content in edible fungi.
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