In this paper,we aimed to select core primers with high polymorphism,strong stability and repetition from a CEAP primer not applied to sugar beet,and to find a suitable method for detecting CEAP primer polymor-phism.In this study,four CEAP molecular markers of cis elements,GATAA,TGAC,AAAG and ACGTG,closely related to plant growth development,signal transduction and stress response,were selected to amplify the genome of some beet varieties and screen 83 CEAP primer polymorphisms by using appropriate detection meth-ods.The results show that the optimal detection method is 2%agarose gel;17 primers from 83 CEAP primers,the polymorphism information content ranges from 0.2608 to 0-0.8446,the average is 0.6649;indicating that CEAP primers have high polymorphism,which can be used for the construction of beet fingerprint and genetic di-versity analysis.